Non-aggregated fluorescent conjugates and the process for their

Details Non-aggregated fluorescent conjugates and the process for their Non-aggregated fluorescent conjugates and the process for their

1998-06-10

2000-09-19

Kight, John

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

536 2532, 536 51, 548465, 548467, 548469, 436800, C12Q 100, C07H 2102

Patent

active

061209872

ABSTRACT:
The invention relates to a process for the preparation of a fluorescent conjugate between a carrier molecule possessing at least one amino, hydroxyl, carboxyl and/or sulfhydryl group and a fluorophoric reagent possessing at least one functional group capable of reacting with said amino, hydroxyl, carboxyl and/or sulfhydryl group(s), which consists in bringing said carrier molecule and said fluorophoric reagent into contact with an aqueous solution of a water-soluble macrocycle.
The invention further relates to the conjugates obtained by this process and to their use.

REFERENCES:
patent: 5068227 (1991-11-01), Weinshenker
patent: 5268486 (1993-12-01), Waggoner et al.
patent: 5627027 (1997-05-01), Waggoner
patent: 5661040 (1997-08-01), Huff et al.
International Search Report dated Mar. 11, 1998 issued in PCT Application No. PCT/FR97/02288 filed Dec. 12, 1997.

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