Nodavirus-like DNA expression vector and uses therefor

Details Nodavirus-like DNA expression vector and uses therefor Nodavirus-like DNA expression vector and uses therefor

2005-03-22

2005-03-22

Guzo, David (Department: 1636)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S320100, C435S235100, C435S069100, C435S325000, C435S348000, C435S455000, C435S456000, C435S091100, C435S091310, C435S091300, C435S091320, C435S091400, C536S023100, C536S023200, C536S023500, C536S023720, C536S024100, C536S024500

Reexamination Certificate

active

06869780

ABSTRACT:
The present invention describes the production of a nodavirus-based DNA vector that drives abundant expression of foreign genes in a wide variety of cell types. The DNA plasmid is initially transcribed by a host-cell RNA polymerase to produce primary transcripts from which a nodaviral RNA-dependent RNA polymerase (RNA replicase) is translated. These primary transcripts are then amplified by the RNA replicase in an autonomous, cytoplasmic RNA replication. Such a vector is a useful addition to the current arsenal of expression vectors, and well suited to laboratory-scale and larger-scale expression of transcripts and/or proteins in eukaryotic cells.

REFERENCES:
Ball, J. Virol., 1992, vol. 66, No. 4, pp. 2335-2345.*
Eckerle et al., Virology, 2002, vol. 296, pp. 165-176.

Affiliated with

Also associated with

Rating

Nodavirus-like DNA expression vector and uses therefor does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Nodavirus-like DNA expression vector and uses therefor, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Nodavirus-like DNA expression vector and uses therefor will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3451090