Nitroxide labeled nucleotides and nitroxide labeled hybridizatio

Details Nitroxide labeled nucleotides and nitroxide labeled hybridizatio Nitroxide labeled nucleotides and nitroxide labeled hybridizatio

1986-02-04

1991-04-02

Brown, Johnnie R.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 22, C07H 1700

Patent

active

050048090

ABSTRACT:
Nitroxide labeled hybridization probes are formed from novel nucleotides. The novel nucleotides are formed by substituting a nitroxide moiety at the C-5 position of the pyrimidine ring of either cytosine or uracil. The hybridization probes are formed by nick translation with E. coli polymerase I or T4 DNA polymerase or other template dependent enzymes. The hybridization probes can then be detected using electron spin resonance spectroscopy or alternately using a colorimetric method.

REFERENCES:
Toppin et al., The Chemical Abstracts, 106:176793g (1987).
Pauly et al., The Chemical Abstracts, 107:231607x (1987).
Kao et al., Biochemistry 24, pp. 5465-5469 (1985).
Enzymatic Synthesis of Biotin-Labeled Polynucleotides: Novel Nucleic Acid Affinity Probes; Pennina R. Langer, Alex. A. Waldrop & David Ward; vol. 78, No. 11, pp. 6633-6637, Nov. 1981, Dept. of Human Genetics & Molecular Biophysics-Biochemistry, Yale University, New Haven, Connecticut.
Dipsticking the Major Groove of DNA with Enzymatically Incorporated Spin-Labeled Deoxyuridines by Electron Spin Resonance Spectroscopy; Albert M. Bobst, Shih-Chung Kao, Roland C. Toppin, John C. Ireland and Ingrid E. Thomas, reprinted from J. Mol. Biol. (1984), 173, 63-74, copyrighted 1984, Academic Press Inc. (London) Ltd.

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