Nitro-substituted non-fluorescent asymmetric cyanine dye...

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

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C536S025300, C536S026600, C548S100000, C548S120000, C548S146000, C548S148000, C548S181000, C548S215000, C548S217000, C548S223000, C435S006120

Reexamination Certificate

active

10840189

ABSTRACT:
The invention provides an asymmetric cyanine dye compound having the structureincluding substituted forms thereof, wherein, at least one of R1and R2is linking group, X is O, S, or Se, and n ranges from 0 to 2. The invention further provides reporter-quencher dye pairs comprising the asymmetric cyanine dyes, dye-labelled polynucleotides incorporating the asymmetric cyanine dyes, and hybridization detection methods utilizing the dye-labelled polynucleotides.

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