Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing nitrogen-containing organic compound
Patent
1997-12-15
1999-06-08
Wax, Robert A.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing nitrogen-containing organic compound
435 691, 435232, 4353201, 435325, 4352523, 43525411, 435410, 536 232, C12P 1302, C12N 988, C12N 1560, C12N 1563, C12N 100, C12N 510
Patent
active
059104325
ABSTRACT:
The present invention provides the amino acid sequence and base sequence of a Pseudonocardia thermophila-derived nitrile hydratase, provides further a method for changing its amino acid sequence and base sequence without substantially changing the functions of said nitrile hydratase, and nitrile hydratases having a base sequence and an amino acid sequence as changed on the basis of said method, and provides furthermore a recombinant plasmid having the gene of said nitrile hydratase, a transformant containing said recombinant plasmid, a method of using said transformant for producing said enzyme, and a method of using said transformant for producing the corresponding amide compound from a nitrile compound.
REFERENCES:
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Enzymatic synthesis of acrylamide: a success story not yet over (TIBTECH
J.A. Wells, "Additivity of mutational effects in proteins", Biochemistry, 29(37):8509-8517 (1990).
Bowie et al, "Deciphering the message in protein sequences: tolerance to amino acid substitutions", Science, 247:1306-1310 (1990).
Mayaux et al., "Purification, Cloning, and Primary Structure of a New Enantiomer-Selective Amidase from a Rhodococcus Strain: Structural Evidence for a Conserved Genetic Coupling with Nitrile Hydratase," Jnl. of Bacteriology, vol. 173, No. 21, Nov. 1991, pp. 6694-6704.
Yamaki et al., "Cloning and Sequencing of a Nitrile Hydratase Gene from Pseudonocardia thermophila JCM3095," Jnl. of Fermentation and Bioengineering, vol. 83, No. 5, 1997, pp. 474-477.
Arii Teruo
Ito Kiyoshi
Nakamura Takeshi
Tsuruoka Miyuki
Yamaki Toshifumi
Bugaisky Gabriele E.
Mitsui Chemicals Inc.
Wax Robert A.
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