Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Lyase
Patent
1997-02-14
1998-09-15
Wax, Robert A.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Lyase
435822, C12N 988
Patent
active
058077308
ABSTRACT:
The present invention provides the amino acid sequence and base sequence of a Pseudonocardia thermophila-derived nitrile hydratase, provides further a method for changing its amino acid sequence and base sequence without substantially changing the functions of said nitrile hydratase, and nitrile hydratases having a base sequence and an amino acid sequence as changed on the basis of said method, and provides furthermore a recombinant plasmid having the gene of said nitrile hydratase, a transformant containing said recombinant plasmid, a method of using said transformant for producing said enzyme, and a method of using said transformant for producing the corresponding amide compound from a nitrile compound.
REFERENCES:
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Enzymatic synthesis of acrylamide: a success story not yet over (TIBTECH
Mayaux et al., "Purification, Cloning, and Primary Structure of a New Enantiomer-Selective Amidase from a Rhodococcus Strain: Structural Evidence for a Conserved Genetic Coupling with Nitrile Hydratase," Jnl. of Bacteriology, vol. 173, No. 21, Nov. 1991, pp. 6694-6704.
Yamaki et al., "Cloning and Sequencing of a Nitrile Hydratase Gene from Pseudonocardia thermophila JCM3095," Jnl. of Fermentation and Bioengineering, vol. 83, No. 5, 1997, pp. 474-477.
J.A. Wells. "Additivity of mutational effects in proteins" Biochemistry 29 (37):8509-8517, Sep. 18, 1990.
Bowie et al. "Deciphering the message in protein sequences: tolerance to amino acid substitutions" Science 247:1306-1310, Mar. 16, 1990.
Arii Teruo
Ito Kiyoshi
Nakamura Takeshi
Tsuruoka Miyuki
Yamaki Toshifumi
Bugaisky Gabriele E.
Mitsui Chemicals Inc.
Wax Robert A.
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