Nitrilases and methods for making and using them

Details Nitrilases and methods for making and using them Nitrilases and methods for making and using them

2007-10-08

2011-10-11

Kam, Chih-Min (Department: 1656)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

C530S350000, C435S106000, C536S023200

Reexamination Certificate

active

08034595

ABSTRACT:
The invention provides nitrilases and methods for making and using them, and in one aspect, provides methods for producing enantiomerically pure α-substituted carboxylic acids, such as, for example, α-amino acids and α-hydroxy acids. In one aspect, methods of the invention combine an aldehyde or ketone with a cyanide and ammonia or an ammonium salt or an amine, in the presence of a nitrilase or a polypeptide having nitrilase activity, to stereoselectively hydrolyze the amino nitrile or cyanohydrin intermediate under conditions sufficient to produce the carboxylic acid.

REFERENCES:
patent: 4611067 (1986-09-01), Volante
patent: 4908313 (1990-03-01), Satoh et al.
patent: 5097045 (1992-03-01), Butler
patent: 5103024 (1992-04-01), Millar
patent: 5124482 (1992-06-01), Butler
patent: 5149837 (1992-09-01), Butler
patent: 5206158 (1993-04-01), Clifford et al.
patent: 5587303 (1996-12-01), Wakamoto et al.
patent: 5635391 (1997-06-01), Petrie
patent: 5756306 (1998-05-01), Yamaguchi et al.
patent: 5872000 (1999-02-01), Yu
patent: 5939553 (1999-08-01), Reichwein
patent: 6042824 (2000-03-01), Khalaf
patent: 2003/0044800 (2003-03-01), Connelly
patent: 0 332 379 (1989-09-01), None
patent: 0332379 (1989-09-01), None
patent: 0 449 648 (1991-10-01), None
patent: 0449648 (1991-10-01), None
patent: 0 502 476 (1992-09-01), None
patent: 0502476 (1992-09-01), None
patent: 0 596 812 (1994-05-01), None
patent: 0596812 (1994-05-01), None
patent: 0 610 048 (1994-08-01), None
patent: 0 610 049 (1994-08-01), None
patent: 0610048 (1994-08-01), None
patent: 0610049 (1994-08-01), None
patent: 0 711 836 (1996-05-01), None
patent: 0711836 (1996-05-01), None
patent: 0 773 297 (1997-05-01), None
patent: 0773297 (1997-05-01), None
patent: 0 780 471 (1997-06-01), None
patent: 0780471 (1997-06-01), None
patent: 61-162195 (1985-01-01), None
patent: 61162195 (1986-07-01), None
patent: 63-500004 (1988-01-01), None
patent: 01-317392 (1989-12-01), None
patent: 01317392 (1989-12-01), None
patent: 04-079894 (1992-03-01), None
patent: 04-099495 (1992-03-01), None
patent: 04079894 (1992-03-01), None
patent: 04099495 (1992-03-01), None
patent: 06-237789 (1994-08-01), None
patent: 06237789 (1994-08-01), None
patent: 08-131188 (1996-05-01), None
patent: 08131188 (1996-05-01), None
patent: 63500004 (1998-01-01), None
patent: 86/07386 (1986-12-01), None
patent: WO-86/07386 (1986-12-01), None
patent: 91/02071 (1991-02-01), None
patent: WO-91/02071 (1991-02-01), None
patent: 91/08287 (1991-06-01), None
patent: 91/08316 (1991-06-01), None
patent: WO-91/08287 (1991-06-01), None
patent: WO-91/08316 (1991-06-01), None
patent: 94/24305 (1994-10-01), None
patent: WO-94/24305 (1994-10-01), None
patent: 97/21805 (1997-06-01), None
patent: WO-97/21805 (1997-06-01), None
patent: 98/13119 (1998-04-01), None
patent: WO-98/13119 (1998-04-01), None
patent: 99/55310 (1999-11-01), None
patent: WO-99/55310 (1999-11-01), None
patent: 00/03685 (2000-01-01), None
patent: WO-00/03685 (2000-01-01), None
patent: 00/58504 (2000-10-01), None
patent: WO-00/58504 (2000-10-01), None
patent: 01/04327 (2001-01-01), None
patent: 01/04331 (2001-01-01), None
patent: WO-01/04327 (2001-01-01), None
patent: WO-01/04331 (2001-01-01), None
patent: 01/14561 (2001-03-01), None
patent: 01/18211 (2001-03-01), None
patent: 01/21782 (2001-03-01), None
patent: WO-01/14561 (2001-03-01), None
patent: WO-01/18211 (2001-03-01), None
patent: WO-01/21782 (2001-03-01), None
patent: 01/25438 (2001-04-01), None
patent: 01/29241 (2001-04-01), None
patent: WO-01/25438 (2001-04-01), None
patent: WO-01/29241 (2001-04-01), None
patent: WO01/48175 (2001-07-01), None
patent: 02/29079 (2002-04-01), None
patent: WO-02/29079 (2002-04-01), None
patent: WO02/29079 (2002-04-01), None
patent: 03/00840 (2003-01-01), None
patent: 03/04456 (2003-01-01), None
patent: 03000840 (2003-01-01), None
patent: WO2004/096988 (2004-11-01), None
Guo et al., Angew Chem. Int. Ed. (1999) 38(12):1755-1758.
Guo et al., PNAS USA (2004) 101:9205-9210.
Hughes et al., “Application of whole cell rhodococcal biocatalysts in acrylic polymer manufacture” Antonie Van Leeuwenhoek (1998) 74, Abstract, Jul.-Oct.
Iyer et al., Amino Acids (1996) 11(304):259-268.
Kim et al., Enzyme and Microbial Tech. (2000) 27:492-501.
Kobayashi et al., Curr. Op. in Chem. Biol. (2000) 4:95-102.
Kobayashi et al., Eur. J. Biochem. (1989) 182:349-356.
Kobayashi et al., FEBS Lett. (1998) 439(3):325-328.
Komeda et al., PNAS USA (1996) 93(20):10572-10577.
Levy-Schil et al., Gene (1995) 161(1):15-20.
Liu et al., Anal. Chem. (1995) 67, Abstract.
Mala et al., Carbo. Rsrch. (1999) 322:209-218.
Martino et al., Proc. Biochem. (1996) 31(3):281-285.
Nagasawa et al.,Tibtech (1989) 7:153-158.
Novo et al., Febs Letts (1995) 367(3):275-279.
Ogawa et al., Trends in Biotech (1999) 17(1):13-20.
Reetz et al., Angew Chem. Int. Ed. (1999) 38(12):1758:1761.
Reetz, Angew Chem. Int. Ed. (2002) 41(8):1335-1338.
Robertson et al., Applied and Environ. Microbiol. (2004) 70(4):2429-2436.
Rouhi, Cenear (2002) 80(23):51-57.
Schrader et al., Can. J. Chem. (2002) 80:626-632.
Shen et al., Israel J. of Chem. (2001) 41:313-316.
Taillades et al., Bulletin De La Societe Chimique De France (1991) 128(3):423-430.
Tao et al., Anal. Chem. (2002) 74:3783-3789.
Wanatabe et al., Applied Microbiol. and Biotech. (1998) 50(1):93-97.
Wiyakrutta et al., J. of Biotech. (1997) 55(3):193-203.
Wu et al., Anal. Letts (1997) 30(7):1399-1406.
Zhao et al., J. of Chromatog. B (1994) 656:441-446.
Zhou et al., “Nucleotide sequence of a pathogen induced nitrilase gene fromArabidopsis thaliana: Nit2 (Accession No. U47114)” Plant Gene Register PGR 96-006 (1995).
EP 08075753.7—Extended European Search Report—Jun. 25, 2009.
PCT/US2008/55569-ISR & WO-Oct. 14, 2008.
EP08731180—Extended European Search Report—Jul. 2, 2010.
EP03753097—Supplementary Partial EP Search Report—Sep. 21, 2007.
EP03753097—EP Office Action—Aug. 11, 2010.
EP10177452—Extended EP Search Report—Nov. 5, 2010.
EPO—Oct. 13, 2010—94(3) Communication—00989578.
Desantis—Journal of the American Chemical Society (2002)-124-9024-9026.
USPTO—Dec. 12, 2007—Office Action—10/514,004.
USPTO—Feb. 3, 2009—Final Office Action—10/514,004.
Robertson—Applied and Environmental Microbiology (2004)-70-2429-2436.
Abato et al., J. Am. Chem. Soc. (2001) 123:9206-9207.
Almatawah et al., Enzyme and Microbial. Tech. (1999) 25:718-724.
Baumann et al., Poster P-130, presented at Bio Trans 2001, Sep. 2-7, 2001, Dramstadt, Germany.
Bhalla et al., App. Micro Biotech (1992) 37:184-190.
Bork et al., Protein Science (1994) 3(8):1344-1346.
Business Communications Company, Amino Acids for Synthesis Applications—Introduction, Summary, Overview, Industry, Manufacture of Amino Acids, Peptide Synthesis Technologies and Amino Acid Products for Synthesis Use Section 7.3 Unnatural Amino Acids, Feb. 1999, 9 pgs.
Business Communications Company, Amino Acids for Synthesis Applications—Introduction, Summary, Overview, Industry, Manufacture of Amino Acids, Peptide Synthesis Technologies and Amino Acid Products for Synthesis Use Section 7.2.5 Prices of Natural Amino Acids, (1999).
Caruso et al., Physicochem. and Engineering Aspects (2000) 169:287-293.
Cheong et al., Enzyme and Microbial Tech. (2000) 26:152-158.
Choi et al., Arch. Pharm. Res. (1986) pp. 45-47.
Cowan et al., Extremophiles (1998) 2:207-216.
Crosby et al., Tetrahedron Asymmetry (1992) 3(12):1547-1550.
Database WPI Section Ch, Week 199217, Derwent Publications Ltd., London, GB; Class B05, AN 1992-137926, XP002165677.
Diaz et al., Tetra Letters (2001) 42:2617-2619.
Dufour et al., FEBS Letters (1998) 433:78-82.
Duran et al., Biosci. Biotech. Biochem. (1993) 57(8):1323-1328.
Fournand et al., J. of Mole. Catalysis B: Enzymatic (1998) 5:207-211.
Gabriel et al., J. of Chromato. B (1996) 681:191-195.
Gallifuoco et al., Process Biochem. (1999) 35:179-185.
GenBank Accession No. E01313, Sep. 29, 1997.
Graham et al., Enzyme and Microbial T

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