Nitrilases and methods for making and using them

Details Nitrilases and methods for making and using them Nitrilases and methods for making and using them

2011-08-09

2011-08-09

Pak, Yong (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

C435S004000, C435S006120, C435S252300, C435S320100, C435S440000, C435S069100, C435S071100, C536S023100, C536S023200, C530S350000

Reexamination Certificate

active

07993901

ABSTRACT:
The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.

REFERENCES:
patent: 4611067 (1986-09-01), Volante
patent: 4908313 (1990-03-01), Satoh et al.
patent: 5003080 (1991-03-01), Butler
patent: 5097045 (1992-03-01), Butler
patent: 5103024 (1992-04-01), Millar
patent: 5124482 (1992-06-01), Butler
patent: 5149837 (1992-09-01), Butler
patent: 5206158 (1993-04-01), Clifford et al.
patent: 5216174 (1993-06-01), Butler
patent: 5245047 (1993-09-01), Butler
patent: 5248793 (1993-09-01), Millar
patent: 5280126 (1994-01-01), Butler
patent: 5298627 (1994-03-01), Butler
patent: 5342952 (1994-08-01), Butler
patent: 5397792 (1995-03-01), Butler
patent: 5446054 (1995-08-01), Butler
patent: 5470981 (1995-11-01), Butler
patent: 5489690 (1996-02-01), Butler
patent: 5489691 (1996-02-01), Butler
patent: 5510488 (1996-04-01), Butler
patent: 5587303 (1996-12-01), Wakamoto et al.
patent: 5629190 (1997-05-01), Petre
patent: 5635391 (1997-06-01), Petre
patent: 5756306 (1998-05-01), Yamaguchi et al.
patent: 5811515 (1998-09-01), Grubbs et al.
patent: 5872000 (1999-02-01), Yu
patent: 5939553 (1999-08-01), Reichwein
patent: 6042824 (2000-03-01), Khalaf
patent: 6433213 (2002-08-01), Bosch
patent: 6596879 (2003-07-01), Bosch
patent: 2002/0127609 (2002-09-01), Davis et al.
patent: 2003/0044800 (2003-03-01), Connelly
patent: 0332379 (1989-09-01), None
patent: 0449648 (1991-10-01), None
patent: 0502476 (1992-09-01), None
patent: 0178106 (1993-03-01), None
patent: 0596812 (1994-05-01), None
patent: 0610048 (1994-08-01), None
patent: 0610049 (1994-08-01), None
patent: 0711836 (1996-05-01), None
patent: 0773297 (1997-05-01), None
patent: 0780471 (1997-06-01), None
patent: 61-162195 (1985-01-01), None
patent: 63-500004 (1988-01-01), None
patent: 1-317392 (1989-12-01), None
patent: 4-079894 (1992-03-01), None
patent: 4-099495 (1992-03-01), None
patent: 6-237789 (1994-08-01), None
patent: 8-131188 (1996-05-01), None
patent: WO-86/07386 (1986-12-01), None
patent: WO-91/02071 (1991-02-01), None
patent: WO-91/08287 (1991-06-01), None
patent: WO-91/08316 (1991-06-01), None
patent: WO-94/24305 (1994-10-01), None
patent: WO-97/21805 (1997-06-01), None
patent: WO-98/13119 (1998-04-01), None
patent: WO-99/55310 (1999-11-01), None
patent: WO-00/03685 (2000-01-01), None
patent: WO-00/58504 (2000-10-01), None
patent: WO-01/04327 (2001-01-01), None
patent: WO-01/04331 (2001-01-01), None
patent: WO-01/14561 (2001-03-01), None
patent: WO-01/18211 (2001-03-01), None
patent: WO-01/21782 (2001-03-01), None
patent: WO-01/25438 (2001-04-01), None
patent: WO-01/29241 (2001-04-01), None
patent: 01/48175 (2001-07-01), None
patent: WO-01/48175 (2001-07-01), None
patent: WO-02/29079 (2002-04-01), None
patent: 03/000840 (2003-01-01), None
patent: WO-03/000840 (2003-01-01), None
patent: WO-03/004456 (2003-01-01), None
patent: WO2004/096988 (2004-11-01), None
Branden et al. Introduction to Protein Structure, Garland Publishing Inc., New York, p. 247, 1991.
EP08731180—Extended European Search Report—Jul. 2, 2010.
EP03753094—Article 94(3) Office Action—Aug. 11, 2010.
EP03753097—Rule 46(1) EPC Supplementary Partial EP Search Report—Sep. 21, 2007.
Abato et al., J Am Chem Soc (2001) 123:9206-9207.
Almatawah et al., Enzyme and Microbial Technology (1999) 25:718-724.
Baumann et al., A high-throughput screening method for the identification of active and enantioselective hydrolases, Poster P-130, presented at Bio Trans 2001, Sep. 2-7, 2001, Dramstadt, Germany.
Baumann et al., Tetrahedron Letters (1992) 33:2283-2284.
Bhalla et al., Applied Micro Biotech (1992) 37:184-190.
Bork et al., Protein Science (1994) 3(8):1344-1346.
Branden et al., Introduction to Protein Structure, Garland Publishing Inc., New York, 1991, p. 247.
Brower et al., Tetrahedron Letters (1992) 33:2279-2282.
Business Communications Company, Amino Acids for Synthesis Applications—Introduction, Summary, Overview, Industry, Manufacture of Amino Acids, Peptide Synthesis Technologies and Amino Acid Products for Synthesis Use Section 7.2.5 Prices of Natural Amino Acids—Feb. 1999.
Business Communications Company, Amino Acids for Synthesis Applications—Introduction, Summary, Overview, Industry, Manufacture of Amino Acids, Peptide Synthesis Tehcnologies and Amino Acid Pruducts for Synthesis Use Section 7.3 Unnatural Amino Acids—Feb. 1999, 9 pgs.
Caruso et al., Physicochemical and Engineering Aspects (2000) 169:287-293.
Cheong et al., Enzyme and Microbial Technology (2000) 26:152-158.
Choi et al., Arch Pharm Res (1986) 9(1):45-47.
Cohen et al., Tetrahedron Letters (1990) 31:7223-7226.
Cowan et al., Extremophiles (1998) 2:207-216.
Crosby et al., Tetrohedron Asymmetry (1992) 3(12):1547-1550.
Diaz et al., Tetrahedron Letters (2001) 42:2617-2619.
Dufour et al., FEBS Lett (1998) 433:78-82.
Duran et al., Characterization of Nitrile Hydratase Genes Cloned by DNA Screening fromRhodococcus erythropolis(1993) 57(8):1323-1328.
Fournand et al., Journal of Molecular Catalysis B: Enzymatic (1998) 5:207-211.
Gabriel et al., Journal of Chromatography B (1996) 681:191-195.
Gallifuoco et al., Process Biochemistry (1999) 35:179-185.
Gavagan et al., J. Org. Chem. (1998) 63:4792-4801.
GenBank Accession No. E-01313, Sep. 29, 1997.
Graham et al., Enzyme and Microbial Technology (2000) 26:368-373.
Guo et al., Angew Chem Int Ed (1999) 38(12):1755-1758.
Hughes et al., Application of whole cell rhodococcal biocatalysts in acrylic polymer manufracture, Antonie Van Leeuwenhoek, vol. 74, Abstract, Oct. 1998.
Kim et al., Enzyme Microbiology Technology (2000) 492-501.
Kobayashi et al., Eur J Biochem (1989) 182:349-356.
Kobayashi et al., Tetrahedron (1990) 46:5587-5590.
Komeda et al.., PNAS USA (1996) 93(20):10572-10577.
Levy-Schil et al., Gene (1995) 161(1):15-20.
Liu et al., Determination of Organonitriles Using Enzyme-Based Selectivity Mechanisms. 2. A Nitrilase-Modified Glassy Carbon Microelectrode Sensor for Benzonitrile, Anal Chem (1995), vol. 67, Abstract.
Mala et al., Carbohydrate Research (1999) 322:209-218.
Martino et al., Process Biochemistry (1996) 31(3):281-285.
Nagasawa et al., Microbial transformations of nitriles (1989) 7:153-158.
Novo et al., FEBS Lett (1995) 367(3):275-279.
Ogawa et al., Microbial enzymes: new industrial applications from traditional screening methods, 9 pages, Jan. 1999.
Reetz et al., Angew Chem Int Ed (1999) 38(12):1758-1761.
Reetz, Angew Chem Int Ed (2002) 41(8):1335-1338.
Robertson et al., Applied and Environmental Microbiology (2004) 70(4):2429-2436.
Rouhi, Chemical & Engineering News (2002) 80(23):51-57.
Schrader et al., Can J Chem (2002) 80:626-632.
Sequence Alignment—Yu et al., Feb. 1999.
Shen et al., Israel Journal of Chemistry (2001) 41:313-316.
Stalker et al., J. Biol. Chem. (1988) 263(13):6310-6314.
Taillades et al., Bulletin De La Societe Chimique De France (1991) 128(3):423-430.
Tao et al., Anal Chem (2002) 74(15):3783-3789.
Watanabe et al., Applied Microbiology and Biotechnology (1998) 50(1):93-97.
Wiyakrutta et al., Journal of Biotechnology (1997) 55(3):193-203.
Wu et al., Analytical Letters (1977) 30(7):1399-1406.
Yao et al., Discovery of Asymmetric Catalysts Using Kinetic Resolution/Mass Spectrometry Screening, Abstract. Pap American Chemical Society, 221st ORGN-606, 2001.
Zhao et al., Journal of Chromatography B (1994) 656:441-446.
Zhou et al., Nucleotide sequence of a pathogen induced nitrilase gene fromArabidopsis thaliana: Nit2 (Accession No. U47114) Plant Gene Register PGR 96-006, Mar. 1996.
Final Office Action for U.S. Appl. No. 10/514,004, mailed on Dec. 20, 2007, 25 pages.
International Search Report mailed on Jan. 5, 2006, for PCT Patent Application No. PCT/US03/15712 filed on May 13, 2003, 2 pages.
Supplementary Partial European Search Report for EP 03 75 3097, mailed on Sep. 21, 2007, 4 pages.
Supplementary European Search Report for EP Application No. 02780877.3, mailed on May 8

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