Nitrilase gene

Details Nitrilase gene Nitrilase gene

1996-07-31

1999-02-16

Prouty, Rebecca E.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435 912, 43525233, 536 232, 536 243, C12N 121, C12P 1934, C07H 2104

Patent

active

058720006

ABSTRACT:
The invention relates to a DNA coding for a polypeptide having nitrilase activity with an amino acid sequence as shown in SEQ ID NO:1. As compared with conventional processes, the hydrolysis of nitriles by a nitrilase gene cloned by genetic recombination is expected to drastically improve the catalytic ability of microorganisms because they can be engineered to contain multiple copies of the same gene.

REFERENCES:
Kobayashi, M. et al. "Nitrilase from Rhodococcus rhodochrous J1" Journal of Biological Chemistry (Oct. 15, 1992), vol. 267, No. 29, pp. 10746-10751, Oct. 1992.

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