Nitrate reductases from red algae, compositions and methods...

Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se

Reexamination Certificate

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C435S006120, C435S069100, C435S468000, C435S419000, C435S252300, C435S320100, C435S183000, C530S370000, C536S023600, C800S278000, C800S301000

Reexamination Certificate

active

07834245

ABSTRACT:
The NR enzymes described herein were discovered in the red algae ofPorphyra perforata(PpNR) andPorphyra yezoensis(PyNR). The present invention provides methods and compositions relating to altering NR activity, nitrogen utilization and/or uptake in plants. The invention relates to a method for the production of plants with maintained or increased yield under low nitrogen fertility. The invention provides isolated nitrate reductase (NR) nucleic acids and their encoded proteins. The invention further provides recombinant expression cassettes, host cells, and transgenic plants. Plants transformed with nucleotide sequences encoding the NR enzyme show improved properties, for example, increased yield and growth.

REFERENCES:
Goltz et al., GenEml Database, Accession No. AM270178, J. Biol. Chem., vol. 257, No. 18, pp. 11186-11190, 1982.
Araki, S., et al.; “Some Enzymic Properties of Nitrate Reductase fromPorphyra yezoensisUEDA f. narawaensis MIURA” Bulletin of the Japanese Society of Scientific Fisheries (1979) 45(7):919-924.
Chow, F., et al.; “In vitro assay and light regulation of nitrate reductase in red algaGracilaria chilensis”; Journal of Plant Physiology (2004) 161:769-776; Elsevier Ltd.; Amsterdam, The Netherlands.
Nakamura, Y. and Ikawa, T., et al.; “Purification and Properties of NADH: Nitrate Reductase from the Red AlgaPorphyra yezoensis”; Plant Cell Physiol (1993) 34(8):1239-1249; Oxford University Press, Oxford, UK.
Thomas, T.E. and Harrison, P.J.; “A Comparison of In Vitro and In Vivo Nitrate Reductase Assays in Three Intertidal Seaweeds”; Botanica Marina (1988) 31:101-107; Walter de Gruyter; Berlin, Germany.

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