Ninhydrin reagent for use in amine and amino acid analyses

Details Ninhydrin reagent for use in amine and amino acid analyses Ninhydrin reagent for use in amine and amino acid analyses

1979-10-18

1981-06-23

Gron, Teddy S.

Chemistry: physical processes

Physical processes

Crystallization

23903, 23230M, 23230R, 252408, 210656, C09K 300, G01N 3348, G01N 3122, G01N 3108

Patent

active

042748330

ABSTRACT:
A ninhydrin reagent is provided for use in automatic analyzers which employs sulfolane as the water miscible organic solvent. The solution is buffered with lithium acetate and contains a small amount of hydrindantin as a stabilizer. The reagent is substantially colorless, exhibits great thermal and pH stability, longer shelf life, and in an automatic analyzer results in better signal to noise ratio, flatter base lines and better resolution of peaks.

REFERENCES:
patent: 3399971 (1968-09-01), Kita
patent: 3689221 (1972-09-01), Udenfriend
patent: 3853473 (1974-12-01), Morin et al.
patent: 3912655 (1975-10-01), Shukla et al.
Moore, S., J. Biol. Chem., vol. 243, No. 23, pp. 6281-6283 (1968).
Moore, S., et al. J. Biol. Chem., vol. 211, pp. 893-913 (1954).
Takahashi J. Biochem., vol. 83, p. 57 (1978).
Moore, S., et al. J. Biol. Chem., vol. 192, pp. 663-681 (1951).
Moore, S., et al. J. Biol. Chem., vol. 176, p. 367 (1948).

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