Chemistry: analytical and immunological testing – Peptide – protein or amino acid
Reexamination Certificate
2007-07-03
2010-12-28
Gakh, Yelena G (Department: 1797)
Chemistry: analytical and immunological testing
Peptide, protein or amino acid
C568S327000
Reexamination Certificate
active
07858376
ABSTRACT:
The invention relates to a method and reagent mixture for the staining and thus visualization of amino acids, peptides and similar compounds, in particular after separation by means of thin-layer chromatography. The staining is carried out using ninhydrin for the detection of amino acids, peptides or proteins in combination with at least one ionic liquid.
REFERENCES:
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Yen (“Synthesis of tetrahydro-b-carbolinediketopiperazines in [bdmim][PF6] ionic liquid accelerated by controlled microwave heating,” Tetrahedron Lett. 2004, 45, 8137-8140).
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Aldrich Handbook of Fine Chemicals and Laboratory Equipment, 2003-2004 edition., Wilwaukee, Wisconsin., pp. 1000, 1337, and T141.
Baczek et al. “Behavior of Peptides and Computer-Assisted Optimization of Peptides Separations in a Normal-Phase Thin-Layer Chromatography System with and Without the Addition of Ionic Liquid in the Eluent.” XP002454070 BiomedicalChromatography 19.1 (2005): 1-8.
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Zabet-Moghaddam et al. “Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry for the Characterization of Ionic Liquids and the Analysis of Amino Acids, Peptides and Proteins in Ionic Liquids.” XP002454071 Journal of Mass Spectrometry 39.12 (2004): 1494-1505.
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Hauck Heinz-Emil
Schulz Michael
Adams Michelle M
Gakh Yelena G
Merck Patent Gesellschaft
Millen White Zelano & Branigan P.C.
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