Nicking of DNA using boronated nucleotides

Details Nicking of DNA using boronated nucleotides Nicking of DNA using boronated nucleotides

1996-08-22

1997-12-30

Horlick, Kenneth R.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435174, 435 911, 536 253, C12P 1934, C12N 1100, C07H 2100

Patent

active

057029261

ABSTRACT:
.alpha.-Boronated deoxynucleoside triphosphates, when incorporated into a double-stranded restriction endonuclease recognition/cleavage site for a restriction endonuclease, induce nicking by the restriction endonuclease. .alpha.-Boronated deoxynucleoside triphosphates (dNTP.alpha.BH.sub.3) are therefore useful as nucleotide analogs in SDA to produce the nickable hemimodified restriction endonuclease recognition/cleavage site required to sustain the amplification reaction.

REFERENCES:
patent: 5270184 (1993-12-01), Walker et al.
patent: 5434143 (1995-07-01), Spielvogel
patent: 5455166 (1995-10-01), Walker

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