Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2005-04-05
2005-04-05
Myers, Carla J. (Department: 1634)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S004000, C435S007100, C435S007920, C435S015000, C435S021000, C536S023100, C536S023500, C530S350000
Reexamination Certificate
active
06875571
ABSTRACT:
The invention provides novel polypeptides which are associated with the transcription complex NF-AT, polynucleotides encoding such polypeptides, antibodies which are reactive with such polypeptides, polynucleotide hybridization probes and PCR amplification probes for detecting polynucleotides which encode such polypeptides, transgenes which encode such polypeptides, homologous targeting constructs that encode such polypeptides and/or homologously integrate in or near endogenous genes encoding such polypeptides, nonhuman transgenic animals which comprise functionally disrupted endogenous genes that normally encode such polypeptides, and transgenic nonhuman animals which comprise transgenes encoding such polypeptides. The invention also provides methods for detecting T cells (including activated T cells) in a cellular sample, methods for treating hyperactive or hypoactive T cell conditions, methods for screening for immunomodulatory agents, methods for diagnostic staging of lymphocyte differentiation, methods for producing NF-AT proteins for use as research or diagnostic reagents, methods for producing antibodies reactive with the novel polypeptides, and methods for producing transgenic nonhuman animals. Also included are methods and agents for activation of NF-AT dependent transcription, including agents which interfere with the production, modification of nuclear or cytoplasmic subunits, or the nuclear import of the cytoplasmic subunits. In particular, screening tests for novel immunosuppressants are provided based upon the ability of NF-AT to activate transcription.
REFERENCES:
patent: 5656452 (1997-08-01), Rao et al.
patent: 5837840 (1998-11-01), Crabtree et al.
patent: 6096515 (2000-08-01), Crabtree et al.
patent: 6150099 (2000-11-01), Crabtree et al.
patent: 6171781 (2001-01-01), Crabtree et al.
patent: 6352830 (2002-03-01), Crabtree et al.
patent: WO 9304203 (1993-03-01), None
patent: WO 9415964 (1994-07-01), None
patent: WO 9502053 (1995-01-01), None
patent: WO 9508554 (1995-03-01), None
Emmel et al., “Cyclosporin A Specifically Inhibits Function of Nuclear Proteins Involved in T Cell Activation”,Reports, Dec. 22, 1989, pp. 1617-1619.
Banerji, S. et al. The immunosuppressant FK-506 specifically inhibits mitogen-induced activation of the interleukin-2 promoter and the isolated enhancer elements NFIL-2A and NF-ATI.Mol. Cell. Biol. 11, 4074-4087 (1991).
Bierer, B. et al. Two distinct signal transmission pathways in T lymphocytes are inhibited by complexes formed between an immunophilin and either FK506 or rapamycin.PNAS87, 9231-9235 (1990).
Clipstone, N. & Crabtree. G. Calcineurin is a key signaling enzyme in T lymphocyte activation and the target of the immunosuppressive drugs cyclosporin A and FK506.Am. N. Y. Acad. Sci. 696, 20-30 (1993).
Clipstone, N. & Crabtree, G. Identification of calcineurin as a key signaling enzyme in lymphocyte activation.Nature357, 695-697 (1992).
Crabtree, G. Contingent genetic regulatory events in T lymphocyte activation.Science243, 355-361 (1989).
Crabtree, G. Pathways of T lymphocyte activation.Abstract of NIH Grant No. R01CA39612(1998).
Crabtree, G. Pathways of T lymphocyte activation.Abstract of NIH Grant No. R01CA39612(1991).
Crabtree, G. & Clipstone, N. Signal transmission between plasma membrane and nucleus of T lymphocyte.Ann. Rev. Biochem. 63, 1045-1083 (1994).
Durand, D. et al. Characterization of antigen receptor response elements within the interleukin-2 enhancer.Mol. Cell. Biol. 8, 1715-1724 (1988).
Emmel, E. et al. Nuclear association of a T-Cell transcription factor blocked by FK-506 and cyclosporin A.Nature352, 803-807 (1991).
Flanagan, W. et al. Nuclear association of a T-cell transcription factor blocked by FK506 and cyclosporin A.Nature352, 803-807 (1991).
Ho, S. et al. Cloning and characterization of NF-ATc and NF-ATp: the cytoplasmic components of NF-AT.Adv. Exp. Med. Biol. 365, 167 (1994).
Israel, A. NF-AT comes under control.Nature369, 443-444 (1994).
Jain et al. Analysis of the preexisting and nuclear forms of nuclear factor of activated T cells.J. Immunol. 151, 837-848 (1993).
Jain, J. et al. Nuclear factor of activated T cells contains Fos and Jun.Nature356, 801-804 (1992).
Jain et al. The T cell transcription factor NF-ATp is a substrate for calcineurin and interacts with Fos and Jun.Nature365, 352-355 (1993).
Jin, Y. et al. Molecular cloning of a membrane-associated human FK-506 and rapamycin-binding protein FKBP-13.PNAS88, 6677-6681 (1991).
Matilla, P. et al. The actions of cyclosporin A and FK506 suggest a novel step in the activation of T lymphocytes.EMBO J. 9, 4425-4433 (1990).
McCaffrey et al. Isolation of the cyclosporin-sensitive T cell transcription factor NF-ATp.Science262, 750-754 (1993).
McCaffrey et al. NF-ATp, a T lymphocyte DNA-binding protein that is a target for calcineurin and immunosuppressive drugs.J. Biol. Chem. 268, 3747-3752 (1993).
Northrop et al. Characterization of the nuclear and cytoplasmic components of the lymphoid-specific nuclear factor of activated T cells (NF-AT) complex.J. Biol. Chem. 268, 2917-2923 (1993).
Northrop et al. NF-AT components define a family of transcription factors targeted in T-Cell Activation.Nature369, 497-502 (1994).
Rao, A. NF-AT: a transcription factor required for the co-ordinate Induction of several cytokine genes.Immunology Today15, 274-281 (1994).
Rao et al. U.S. Appl. No. 08/145,006, filed May 11, 1993
Rao et al. U.S. Appl. No. 08/017,052 filed Feb. 11, 1993.
Rao et al. U.S. Appl. No. 08/006,067 filed Jan. 15, 1993.
Riegel, J. et al. Nuclear Events after activation of CD4+8+ thymocytes.J. Immunol. 144, 6-3611-3618 (1990).
Shaw, J. et al. Identification of a putative regulator of early T cell activation genes.Science241, 202-225 (1988).
Schmidt, A. et al. Inducible Nuclear Factor Binding to the kB elements of the human immunodeficiency virus enhancer in T cells can be blocked by cyclosporin A in a signal-dependent manner.J. Virology64, 4037-4041 (1990).
Schreiber, S. Chemistry and biology of the immunophilins and their immunosuppressive ligands.Science251, 283-287 (1991).
Verweij, C. et al. Cell type specificity and activation requirements for NF-AT-1 (Nuclear Factor of Activated T-cells) transcriptional activity determined by a new method using transgenic mice to assay transcriptional activity of an individual nuclear factor.J. Biol. Chem. 265, 15788-15795 (Sep. 15, 1990).
Crabtree Gerald R.
Flanagan William M.
Ho Steffan N.
Northrop Jeffrey P.
Myers Carla J.
Ropes & Gray LLP
The Board of Trustees of the Leland Stanford Junior University
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