New restriction enzyme and process for producing the same

Details New restriction enzyme and process for producing the same New restriction enzyme and process for producing the same

1986-09-10

1989-05-23

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435 91, 435823, C12N 922, C12P 1934, C12R 102

Patent

active

048330825

ABSTRACT:
A restriction enzyme, ApaLI, which recognizes the base sequence in a double-stranded deoxyribonucleic acid molecule as shown below, and cleaves it at the arrow-marked sites, ##STR1## wherein A, G, T and C represent adenosine, guanosine, thymidine and cytidine, respectively. Also provided is a process for producing this enzyme, by growing a microorganism belonging to the genus Acetobacter.

REFERENCES:
Yamada, Y., et al. (1985) Agric. Biol. Chem., 49(12) 3627-3629.
Degtyarev. S. Kh. et al. (1987) Chemical Abstracts, 106:209994d.
Roberts, R. J. (1987) Nucleic Acids Research, 15 (suppl.) r189, r190, r215.

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