New elution complex more especially for affinity chromatography

Details New elution complex more especially for affinity chromatography New elution complex more especially for affinity chromatography

1985-09-25

1987-05-19

Therkorn, Ernest G.

Liquid purification or separation

Miscellaneous

210635, 210656, 210672, 210674, 2105001, 2105021, 252 1, 252364, B01D 1508

Patent

active

046666044

ABSTRACT:
A novel elution complex is provided more especially for affinity chromatography and affinity precipitation for recovering in particular luciferase hexokinase and lactate dehydrogenase.
This complex comprises 1 mM to 30 mM of MgSO.sub.4, from 30 mM to 50 mM of EDTA, possibly about 5 mM of ribose or mannose and possibly about 1 mM of DTT.

REFERENCES:
patent: 4409335 (1983-10-01), Hanamoto et al.
Small et al., High-Performance Liquid Affinity Chromatography Of Enzymes On Silica-Immobilised Triazine Dyes; J. Of Chrom. 261 (1981) 175-190.
Dyes-A Colourful Addition To Protein Purification By Yvonne Hey And Peter D. G. Dean; Chemistry & Industry, Oct. 17, 1981.

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