Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1991-09-06
1994-05-31
Saunders, David
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 794, 435975, 436518, 436536, 436548, G01N 33543
Patent
active
053169131
ABSTRACT:
Methods and compositions are provided for determining neutrophil activation in a mammalian host by detecting the presence of shed LECAM-1 in the blood. By employing a monoclonal antibody which binds to a common epitope of lymphocyte and neutrophil LECAM-1 and a monoclonal antibody which binds to a sialylated Lewis X epitope which distinguishes between neutrophil and lymphocyte LECAM-1, the level of neutrophil activation can be determined. Various immunoassay protocols may be employed which detect the binding of the two antibodies to a common antigen.
REFERENCES:
patent: 5006459 (1991-04-01), Kung et al.
"Carbohydrate ligands of the LEC cell adhesion molecules" by Brandley et al. in Cell (1990) 63:861-863.
"Chemotactic factors regulate lectin adhesion molecule 1 (LECAM-1)-dependent neutrophil adhesion to cytokine-stimulated endotholial cells in vitro" by Smith et al. in J. Clin. Invest. (1991) 87:609-618.
"Identification of a human peripheral lymph node homing receptor: A rapidly down-regulated adhesion molecule" by Kishimoto et al. in Proc. Natl. Acad. Sci. USA (1990) 87:224-2248.
"Function and regulation of the neutrophil MEL-14 antigen in vivo: Comparison with LFA-1 and MAC-1." by Jutila et al. in J. Immunol. (1989) 143:3318-3324.
"Cloning of GMP-140, a granule membrane protein of platelets and endothelium: Sequence similarity to proteins involved in cell adhesion and inflammation" by Johnston et al. in Cell (1989) 56:1033-1044.
"The peripheral lymph node homing receptor, LECAM-1 is involved in CD18-independent adhesion of human neutrophils to endothelium" by Hallman et al. in Biochem. Biophys. Res. Comm. (1991) 174:236-243.
"A unique phenotype of skin-associated lymphocytes in humans" by Picker et al. in J. Path. (1990) 136:1053-1068.
"Quantitative investigations of the adhesiveness of the circulating polymorphonuclear leukocytes to blood vessel walls" by Atherton and Born in J. Physiol. (1972) 222:447-474.
"Leukocyte-endothelial cell recognition: Evidence of a common molecular mechanism shared by neutrophils, lymphocytes, and other leukocytes" by Lewinson et al. in J. Immunol. (1987) 138:4313-4321.
"CD11/CD18-independent neutrophil adherence to inducible endothelial-leucocyte adhesion molecules (E-LAM) in vitro" by Dobrina et al. in Immunology (1989) 67:502-508.
"ELAM-1 is an adhesion molecule for skin-homing T cells" by Picker et al. in Nature (1991) 349:796-799.
"The neutrophil selectin LECAM-1 presents carbohydrate ligands to the vascular selectins ELAM-1 and GMP-140" by Picker et al. in Cell (1991) 66:921-933.
"Two-step model of leukocyte-endothelial cell interaction in inflammation:Distinct roles for LECAM-1 and the Leukocyte Beta, integrins in vivo" by von Adrian et al. in Proc. Natl. Acad. Sci. USA (1991) 88:7538-7542.
"Differential expression of homing-associated adhesion molecules by T cell subsets in man" by Picker et al. in J. Immunol. (1990) 145:3247-3255.
F. W. Luscinskas et al, FASEB J5(6), A1602, 1991.
M. Berg et al, Blood, 76, 2381-2388, 1990.
Butcher Eugene C.
Picker Louis J.
Rowland Bertram I.
Saunders David
Stanford University
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