Neutralizing antibodies and methods of use thereof

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...

Reexamination Certificate

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C530S388100, C530S388220, C530S388850, C530S387300, C530S388230, C424S130100, C424S133100, C424S141100

Reexamination Certificate

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11009939

ABSTRACT:
This invention provides monoclonal antibodies that recognize the Toll-like Receptor 4/MD-2 receptor complex, and monoclonal antibodies that recognize the TLR4/MD2 complex as well as TLR4 when not complexed with MD-2. The invention further provides methods of using the monoclonal antibodies as therapeutics. This invention also provides soluble chimeric proteins, methods of expressing and purifying soluble chimeric proteins, and methods of using soluble chimeric proteins as therapeutics, in screening assays and in the production of antibodies.

REFERENCES:
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patent: 2003/0027260 (2003-02-01), Goddard et al.
patent: 2005/0169932 (2005-08-01), Cheung
Kawasaki et al. 2003. Jnl of Immunology. 170:413-420.
Nijhuis et al. 2003. Clinical and Diagnostic Lab Immunol. 10:558-563.
Akashi et al. 2000. Jnl of Immunology 164:3471-3475.
Winter et al. 1985. EMBO J. 4:2861-2867) teach a sequence (amino acids 19-134.

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