Neurospora hosts for the production of recombinant proteins, and

Details Neurospora hosts for the production of recombinant proteins, and Neurospora hosts for the production of recombinant proteins, and

1995-12-15

1998-07-07

Kemmerer, Elizabeth C.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4352544, C12N 1563, C12N 114, C12N 115

Patent

active

057767300

ABSTRACT:
The present invention provides improved host cells for use in producing secreted recombinant proteins, methods of making the improved hosts and uses thereof. The invention specifically provides strains of Neurospora crassa which produce reduced levels of extracellular proteases when compared with wild-type Neurospora crassa.

REFERENCES:
patent: 4486533 (1984-12-01), Lambowitz
patent: 4816405 (1989-03-01), Timberlake et al.
patent: 4885249 (1989-12-01), Buxton et al.
patent: 4935349 (1990-06-01), McKnight et al.
patent: 5179003 (1993-01-01), Wolf et al.
Van den Hondel et al. Heterologous production in filamentous fungi. In: More Genetic Manipulation of Filamentous Fungi. Bennett and Lasure, eds. Academic Press, Orlando, FL. pp. 396-428, 1991.
Archer et al. Strategies for improving heterologous protein production from filamentous fungi. Antonie van Leeuwenhoek. vol. 65, No. 3, pp. 245-250, 1994.
ATCC Catalogue of Filamentous Fungi, 18th Edition. American Type Culture Collection Accession No. 11943. p. 259, 1991.
Dunn-Coleman et al. Commercial levels of chymosin production by Aspergillus. Bio/Technology. vol. 9, No. 10, pp. 976-981, Oct. 1991.
Jeenes et al. Heterologous protein production by filamentous fungi. Biotechnology and Genetic Engineering Reviews. vol. 9, pp. 327-367, 1991.
Jones. Takling the protease problem in Saccharomyces cerevisiae. In: Methods in Enzymology. Guthrie and Fink, eds. Academic Press, Inc., NY. vol. 194, pp. 428-453, 1991.
Heiniger et al. Protease secretion in Neurospora crassa. Biochemical and Biophysical Research Communications. vol. 60, No. 4, pp. 1425-1432, Oct. 23, 1974.
Lawrence. Classical mutagenesis techniques. In: Methods in Enzymology . Guthrie and Fink, eds. Academic Press, Inc., NY. vol. 194, pp. 273-281, 1991.
Polizeli et al. Pectinase production by Neurospora crassa: purification and biochemical characterization of extracellular polygalacturonase activity. Journal of General Microbiology. vol. 137, Pt. 8, pp. 1815-1823, Aug. 1991.
Savtchenko et al. Alkaline protease deficiency in the cr-1 (crisp) mutant of Neurospora crassa. Brazilian Journal of Medical and Biological Research. vol. 19, No. 1, pp. 27-32, 1986.
Vazquez-Laslop et al. Characterization of a vacuolar protease in Neurospora crassaand the use of gene RIPing to generate protease-deficient strains. The Journal of Biological Chemistry. vol. 271, No. 36, pp. 21944-21949, Sep. 6, 1996.
Bennett et al., "More Genetic Manipulation of Filamentous Fungi," Academic Press, 1991, pp. 396-428.
Guthrie et al., "Methods in Enzymology," Academic Press, 1991, vol. 194, pp. 273-281.
Guthrie et al., "Methods in Enzymology," Academic Press, 1991, vol. 194, pp. 428-453.
Mackenzie et al., "Regulation of Secreted Protein Production by Filamentous Fungi: Recent Developments and Perspectives," Journal of General Microbiology, 1993, vol. 139, pp. 2295-2307.
Stuart, W.D. et al. Genome (1988) 30:198-203.
Koo, K. et al. Genome (1991) 34:644-651.
Carattoli, A. et al. Proc Natl Acad Sci USA (1995) 92:6612-6616.
Yamashita, R.A. et al. Fungal Genetics Newsletter (1995 Suppl.) 42A.
Kato, E. et al. Fungal Genetics Newsletter (1995 Suppl.) 42 A.
Buczynski, S. et al. Fungal Genetics Newsletter (1995 Suppl.) 42 A.
Lindberg, R.A. et al. J Bacteriol (1982) 150(3):1103-1108.
Cohen, L. et al. Archiv Biochem Biophys (1975) 169:324-330.
Abbott, R.A. et al. J Bacteriol (1984) 159(2):505-510.
Hanson, M.A. et al. Proc Natl Acad Sci USA (1975) 72(4): 1240-1244.
Peberdy, J.F., Trends in BioTechnology (1974) 12:50-57.

Affiliated with

Also associated with

Rating

Neurospora hosts for the production of recombinant proteins, and does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Neurospora hosts for the production of recombinant proteins, and, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Neurospora hosts for the production of recombinant proteins, and will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1204379