Neuropeptides and their use as insecticides

Details Neuropeptides and their use as insecticides Neuropeptides and their use as insecticides

1995-11-01

1998-05-19

Hutzell, Paula K.

Drug, bio-affecting and body treating compositions

Designated organic active ingredient containing

Peptide containing doai

424405, 514 13, 514 14, 514 16, 514 21, 530326, 530327, 530328, 530412, A01N 2500, A01N 3804, C07K 700, C07K 100

Patent

active

057536155

DESCRIPTION:

BRIEF SUMMARY
CROSS REFERENCE TO RELATED APPLICATIONS

This application is a U.S. national stage application under 35 U.S.C. .sctn. 371 of PCT International Application No. PCT/GB94/00507 filed on Mar. 14, 1994.
Neuropeptides capable of inhibiting the production of juvenile hormone by the corpus allatum of insects have been termed allatostatins. To date, members of this class of neuropeptides have been identified in only two species, from two different orders of insects. In the cockroach, Diploptera punctata (Order: Blattodea) five allatostatins ranging in size from 8 to 18 amino acids have been characterised. In the tobacco hornworm moth, Manduca sexta (Order: Lepidoptera) one allatostatin has been identified.
The References to these compounds together with their amino acid sequences are as follows: structure of four allatostatins: neuropeptide inhibitors of juvenile hormone synthesis. Proc. Natl. Acad. Sci. USA 86: 5997-6001 discloses the following sequences:


__________________________________________________________________________ Ala--Pro--Ser--Gly--Ala--Gln--Arg--Leu--Tyr--Gly--Phe--Gly--Leu--NH.sub. 2 (SEQ ID NO: 1) Gly--Asp--Gly--Arg--Leu--Tyr--Ala--Phe--Gly--Leu--NH.sub.2 (SEQ ID NO: 2) Gly--Gly--Ser--Leu--Tyr--Ser--Phe--Gly--Leu--NH.sub.2 (SEQ ID NO: 3) Asp--Arg--Leu--Tyr--Ser--Phe--Gly--Leu--NH.sub.2 (SEQ ID NO: 4) __________________________________________________________________________ Shabanowitz J, Hunt D F, and Feyereisen R. (1991) Identity of a second type of allatostatin from cockroach brains: An octadecapeptide amide with a tyrosine-rich address. Proc. Natl. Acad, Sci. USA 88: 2412-2416 discloses the following sequence: sub.2 (SEQ ID NO:5); and Carney R L, Schooley D A. (1991). Identification of an allatostatin from the tobacco hornworm Manduca sexta. Proc. Natl. Acad. Sci. USA 88: 9458-9462 discloses the amino acid sequence: NO:6)
The significance of these compounds lies in the fact that "in vitro" they have been shown to inhibit the production of juvenile hormone by the corpus allatum. This hormone plays a crucial role in insect development by controlling metamorphosis, adult sexual maturity and reproduction.
Interference with juvenile hormone biosynthesis and release through exploitation of the allatostatins could lead to insect control strategies that do not damage the environment.
The present invention is based on the discovery and potential practical commercial applications of one or more of five neuropeptides now identified from the blowfly, Calliphora vomitoria. These are the first allatostatins to be isolated from insects belonging to the Order: Diptera.
The amino acid sequences of the newly discovered neuropeptides designated "callatostatins 1-4" are as follows:


__________________________________________________________________________ (1) Asp--Pro--Leu--Asn--Glu--Glu--Arg--Arg--Ala--Asn--Arg--Tyr--Gly--Phe--Gl y--Leu--NH.sub.2 (SEQ ID NO: 7) (2) Leu--Asn--Glu--Glu--Arg--Arg--Ala--Asn--Arg--Tyr--Gly--Phe--Gly--Leu--NH .sub.2 (SEQ ID NO: 8) (3) Ala--Asn--Arg--Tyr--Gly--Phe--Gly--Leu--NH.sub.2 (SEQ ID NO: 9) (4) (Asp)--Arg--Pro--Tyr--Ser--Phe--Gly--Leu--NH.sub.2 (SEQ ID NO: 10) or (Asn) __________________________________________________________________________
The amino acid sequence of the more preferred neuropeptide termed "callatostatin 5" is:
i.e. Glycine-Proline-Proline-Tyrosine-Aspartic Acid-Phenylalanine-Glycine-Methionine-Amide.
"Callatostatin 5," as defined above was isolated from extracts of whole flies. Subsequently, callatostatin 5 has been identified on three separate occasions from acid alcohol extracts of the separated heads of blowflies. Furthermore, it has been confirmed that the final methionyl residue is carboxyamidated by means of standard methylation procedures and comparisons of the masses of methylated and non-methylated peptides.
The extraction of the callatostatins from whole flies and also from separated heads was performed using 80% methanol/0.1M HCL/0.1% 2-mercapto-ethanol or a mixture of 87% methanol

REFERENCES:
patent: 5039792 (1991-08-01), Feyereisen et al.
Donly, B.C., et al., "Molecular cloning of the gene for the allatostatin family of neuropeptides from the cockroach Diploptera punctata," Proc. Natl. Acad. Sci. USA 90:8807-8811 (Oct. 1993).
Duve, H., et al., "Isolation, structure, and activity of -Phe-Met-Arg-Phe-NH.sub.2 neuropeptides (designated calliFMRFamides) from the blowfly Calliphora vomitoria," Proc. Natl. Acad. Sci. USA 89:2326-2330 (Mar. 1992).
Duve, H., et al., "Callatostatins: Neuropeptides from the blowfly Calliphora vomitoria with sequence homology to cockroach allatostatins," Proc. Natl. Acad. Sci. USA 90:2456-2460 (Mar. 1993).
Richard, D.S., et al., "Allatostatic regulation of juvenile hormone production in vitro by the ring gland of Drosophila melanogaster," Molec. Cell. Endocrinol. 68(2-3):153-161 (Jan. 22, 1990).
Woodhead, A.P., et al., "Primary structure of four allatostatins: Neuropeptide inhibitors of juvenile hormone synthesis," Proc. Natl. Acad. Sci. USA 86:5997-6001 (Aug. 1989).
Zitnan, D., et al., "Neurons Producing Specific Neuropeptides in the Central Nervous System of Normal and Pupariation-Delayed Drosophila," Dev. Biol. 156(1):117-135 (Mar. 1993).

Affiliated with

Also associated with

Rating

Neuropeptides and their use as insecticides does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Neuropeptides and their use as insecticides, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Neuropeptides and their use as insecticides will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1852991