Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1997-04-07
1999-10-12
Teng, Sally P.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4352523, 4352542, 4353201, 435325, 435348, 435366, 536 235, 536 2431, C12N 1512
Patent
active
059653922
ABSTRACT:
The present invention provides novel NPY/PYY receptor proteins and nucleic acid sequence encoding them. The invention is directed to the isolation, characterization, and pharmacological use of these receptors and nucleic acids. In particular, this invention provides human and rat NPY/PYY receptors (which we call the NPY Y5 receptor) and nucleic acids. Also provided are recombinant expression constructs useful for transfecting cells and expressing the protein in vitro and in vivo. The invention further provides methods for detecting expression levels of the protein as well as methods for screening for receptor antagonists and agonists to be used for the treatment of obesity or anorexia, respectively.
REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4736866 (1988-04-01), Leder et al.
patent: 5175383 (1992-12-01), Leder et al.
patent: 5602024 (1997-02-01), Gerald et al.
patent: B14683202 (1990-11-01), Mullis et al.
Bertling, "Transfection of a DNA/Protein Complex into Nuclei of Mammalian Cells Using Polyoma Capsids and Electroporation," Bioscience Reports 7:107-112 (1987).
Bottcher et al., "Coexistence of peptide YY and glicentin immunoreactivity in endocrine cells of the gut," Regul. Pept. 8:261 (1984).
Capecchi, "Altering the Genome by Homologous Recombination," Science 244:1288 (1989).
Dumont et al., "Comparative Characterization and Autoradiographic Distribution of Neuropeptide Y Receptor Subtypes in the Rat Brain," The Journal of Neuroscience 13(1):73-86 (Jan., 1993).
Eva et al., "Molecular cloning of a novel G protein-coupled receptor that may belong to the neuropeptide receptor family," FEBS Lett. 271:81 (1990).
Eva et al., "The murine NPY-1 receptor gene. Structure and delineation of tissue-specific expression," FEBS Lett. 314:285 (1992).
Gerald et al., "A receptor subtype involved in neuropeptide-Y-induced food intake," Nature 382:168 (1996).
Glover, "Conformational studies on the pancreatic polypeptide hormone family," Eur. J. Biochem. 142:379 (1985).
Gordon et al., "Characterization of Functional Neuropeptide Y Receptors in a Human Neuroblastoma Cell Line," J. Neurochem. 55:506 (1990).
Gubler and Hoffman, "A simple and very efficient method for generating cDNA libraries," Gene 25:263 (1983).
Hanks et al., "Rescue of the En-1Mutant Phenotype by Replacement of En-1 with En-2 ," Science 269:679 (1995).
Hasty et al., "Introduction of a subtle mutation into the Hox-2.6 locus in embryonic stem cells," Nature 350:243 (1991).
Hazlewood, "The Pancreatic Polypeptide (PP-Fold) Family: Gastrointestinal, Vascular, and Feeding Behavioral Implications," Proc. Soc. Exp. Biol. Med. 202:44 (1993).
Herzog, "Overlapping Gene Structure of the Human Neuropeptide Y Receptors Subtypes Y1 and Y5 Suggests Coordinate Transcriptional Regulation," Genomics 41:315-319 (1997).
Hogan et al., Manipulating the Mouse Embryo, A Laboratory Manual (Cold Spring Harbor Laboratory (1986).
Howlett, "Prime time for neuropeptide Y," Nature 382:113 (1996).
Hu et al., "Identification of a Novel Hypothalamic Neuropeptide Y Receptor Associated with Feeding Behavior," The Journal of Biological Chemistry 271 (42):26315-26319 (1996).
Invitrogen Corporation publications 130813sa and 130928sa.
Kalra et al., "Structure-Function Analysis of Stimulation of Food Intake by Neuropeptide Y: Effects of Receptor Agonists," Phys. & Behavior 50:5 (1991).
Katsuki et al., "Conversion of Normal Behavior to Shiverer by Myelin Basic Protein Antisense cDNA in Transgenic Mice," Science 241:593 (1988).
Kuhn et al., "Inducible Gene Targeting in Mice," Science 269:1427 (1995).
Larhammar et al., "Cloning and Functional Expression of a Human Neuropeptide Y/Peptide YY Receptor of the Y1 Type," J. Biol. Chem. 267:10935 (1992).
Lundell et al., "Cloning of a Human Receptor of the NPY Receptor Family with High Affinity for Pancreatic Polypeptide and Peptide YY," J. Biol. Chem. 270:29123 (1995).
Marx, "Knocking Genes In Instead of Out," Science 269:636 (1995).
Maxam et al., "Sequencing End-Labeled DNA with Base-Specific Chemical Cleavages," Methods in Enzymology 65:499-560 (1980).
Morley et al., "Peptide YY (PYY), a potent orexigenic agent," Brain Res. 341:200 (1985).
Nakamura et al., "Identification of Two Isoforms of Mouse Neuropeptide Y-Y1 Receptor Generated by Alternative Splicing," J. Biol. Chem. 270:30102 (1995).
Okayama and Berg, "High-Efficiency Cloning of Full-Length cDNA," Molec. Cell. Biol. 2:161 (1982).
Rose et al., "Cloning and Functional Expression of a cDNA Encoding a Human Type 2 Neuropeptide Y Receptor," J. Biol. Chem. 270:22661 (1995).
Sambrook et al., Molecular Cloning: A Laboratory Manual (2.sup.nd Edition, Cold Spring Harbor Press, New York, 1989).
Smithies et al., "Insertion of DNA sequences into the human chromosomal .beta.-globin locus by homologous recombination," Nature 317:230-234 (1985).
Stacey et al., "Use of Double-Replacement Gene Targeting To Replace the Murine .alpha.-Lactalbumin Gene with Its Human Counterpart in Embryonic Stem Cells and Mice," Mol. Cell Biol. 14:1009 (1994).
Stanley et al., "Evidence for Neuropeptide Y Mediation of Eating Produced by Food Deprivation and for a Variant of the Y.sub.1 Receptor Mediating This Peptide's Effect," Peptides 31:581-587 (1992).
Tatemoto et al., "Neuropeptide Y--a novel brain peptide with structural similarities to peptide YY and pancreatic polypeptide," Nature 296:659 (1982).
Tatemoto, "Neuropeptide Y: Complete amino acid sequence of the brain peptide," Proc. Natl. Acad. Sci. USA 79:5485 (1982).
Thomas & Capecchi, "Site-Directed Mutagenesis by Gene Targeting in Mouse Embryo-Derived Stem Cells," Cell 51:503-512 (1987).
Zimmer and Gruss, "Production of chimaeric mice containing embryonic stem (ES) cells carrying a homoeobox Hox 1.1 allele mutated by homologous recombination," Nature 338:150 (1989).
Bloomquist Brian T.
Cornfield Linda J.
Flores-Riveros Jaime R.
Hu Yinghe
McCaleb Michael L.
Bayer Corporation
Teng Sally P.
LandOfFree
Neuropeptide Y receptor Y5 and nucleic acid sequences does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Neuropeptide Y receptor Y5 and nucleic acid sequences, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Neuropeptide Y receptor Y5 and nucleic acid sequences will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-651218