Neuronal nicotinic acetylcholine receptor compositions

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues

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435 6, 435 621, 4352523, 4353201, C07K 1300, C12N 1512

Patent

active

053711882

ABSTRACT:
The present invention relates to a family of neuronal nicotinic acetylcholine receptors comprised of neuronal agonist and non-agonist binding subunits, and DNA sequences encoding such subunits. These novel neuronal nicotinic acetylcholine receptor subunits include the agonist binding subunits alpha2, alpha3, alpha4, and alpha5, plus non-agonist binding subunits beta2, beta3 and beta4. Representative cDNA clones that contain the DNA sequences of the invention have been deposited with the American Type Culture Collection for patent purposes.

REFERENCES:
patent: 4518527 (1985-05-01), Numa
Mishina, M. et al. "Expression of Functional Acetylcholine receptor . . . " Nature vol. 307, pp. 604-608 Feb. 16, 1984.
Whiting & Lindstrom, Proc. Natl. Acad. Sci. USA vol. 84, pp. 595-599, Jan. 1987.
Boulter, J. et al. "Isolation of a cDNA clone coding for a possible neural nicotinic acetylcholine receptor .alpha.-subunit" Nature vol. 319 pp. 368-374 Jan. 30, 1986.
Goldman, D. et al. "Members of a Nicotine Acetylcholine receptor gene family . . . " Cell vol. 48, pp. Mar. 27, 1987.
Whiting, P. J. et al. "Functional acetylcholine receptor in PC12 cells . . . " Nature vol. 327 pp. 515-518 Jun. 11, 1987.
Whiting, Paul & Lindstrom, Jon "Affinity Labelling of Neuronal acetylcholine receptors . . . " FEBS Letters vol. 23(1) pp. 55-60 Mar. 1987.

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