Neuronal nicotinic acetylcholine receptor assay

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 6, 435 72, 436501, G01N 3353

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055915906

ABSTRACT:
The present invention relates to a family of neuronal nicotinic acetylcholine receptors comprised of neuronal agonist and non-agonist binding subunits, and DNA sequences encoding such subunits. These novel neuronal nicotinic acetylcholine receptor subunits include the agonist binding subunits alpha2, alpha3, alpha4, and alpha5, plus non-agonist binding subunits beta2, beta3 and beta4. Representative cDNA clones that contain the DNA sequences of the invention have been deposited with the American Type Culture Collection for patent purposes.

REFERENCES:
patent: 4518527 (1985-05-01), Numa et al.
Armstrong et al., "Distribution of Cholinergic Neurons in Rat Brain: Demonstrated by the Immunocytochemical Localizaton of Choline Acetyltransferase" J. Comp. Neurol. 216:53-68 (1983).
Baldwin et al., "Structure, evolution and regulation of a fast skeletal muscle troponin I gene" Proc. Natl. Acad. Sci., USA 82:8080-8084 (1985).
Barnard et al., "Translation of exogenous messenger RNA coding for nicotinic acetylcholine receptors produces functional receptors in Xenopus oocytes" Proc. R. Soc. Lond. B. 215:241-246 (1982).
Boulter et al., "Isolation of a Clone Coding for the .alpha.-Subunit of a Mouse Acetylcholine Receptor" J. Neurosci. 5(9):2545-2552 (1985).
Boulter et al., "Isolation of a cDNA clone coding for a possible neural nicotinic acetylcholine receptor .alpha.-subunit" Nature 319:368-374 (1986).
Boulter et al., "Functional expression of two neuronal nicotinic acetylcholine receptors from cDNA clones identifies a gene family" Proc. Natl. Acad. Sci., USA 84:7763-7767 (1987).
Breitbart and Nadal-Ginard, "Developmentally Induced, Muscle-Specific Trans Factors Control the Differential Splicing of Alternative and Constitutive Troponin T Exons" Cell 49:793-803 (1987).
Brockes and Hall "Synthesis of Acetylcholine Receptor by Denervated Rat Diaphragm Muscle" Proc. Natl. Acad. Sci., USA 72(4):1368-1372 (1975).
Brown and Fumagalli, "Dissociation of .alpha.-bungarotoxin binding and receptor block in the rat superior cervical ganglion" Brain Res. 129:165-168 (1977).
Brown et al., "The action of cholinomimetic substances on impulse conduction in the habenulointerpeduncular pathway of the rat in vitro" J. Physiol. 353:101-109 (1984).
Buonanno and Merlie, "Transcriptional Regulation of Nicotinic Acetylcholine Receptor Genes during Muscle Develoment" J. Biol. Chem. 261(25):11452-11455 (1986).
Carbonetto et al., "Nonequivalence of .alpha.-bungarotoxin receptors and acetylcholine receptors in chick sympathetic neurons" Proc. Natl Acad. Sci., USA 75(2):1016-1020 (1978).
Chiappinelli and Dryer, "Nicotinic transmission in sympathetic ganglia: blockade by the snake venom neurotoxin kappa-bungarotoxin" Neurosci. Lett. 50:239-244 (1984).
Clarke et al., "Nicotinic Binding in the Rat Brain: Autoradiographic Comparison of [.sup.3 H]Acetylcholine, [.sup.3 H]Nicotine, and [.sup.125 H]-.alpha.-Bungarotoxin" J. Neurosci. 5(5):1307-1315 (1985).
Claudio et al., "Nucleotide and deduced amino acid sequences of Torpedo californica acetylcholine receptor .gamma. subunit" Proc. Natl. Acad. Sci., USA 80:1111-1115 (1983).
Conti-Tronconi et al., "Brain and muscle nicotinic acetylcholine receptors are different but homologous proteins" Proc. Natl. Acad. Sci., USA 82:5208-5212 (1985).
Cox et al., "Detection of mRNAs in Sea Urchin Embryos by in Situ Hybridizaton Using Aasymmetric RNA Probes" Dev. Biol. 101:485-502 (1984).
Deneris et al., "Primary Structure and Expression of .beta.2: A novel Subunit of Neuronal Nicotinic Acetylcholine Receptors" Neuron 1:45-54 (1988).
Devillers-Thiery et al., "Complete mRNA coding sequence of the acetylcholine binding .alpha.-subunit of Torpedo marmorata acetylcholine receptor: A model for the transmembrane organization of the polypeptide chain" Proc. Natl Acad. Sci., USA 80:2067-2071 (1983).
Evans et al., "Muscle Acetylcholine Receptor Biosynthesis: Regulation by Transcript Availability" J. Biol. Chem. 262(10):4911-4916 (1987).
Finer-Moore and Stroud, "Amphipathic analysis and possible formation of the ion channel in an acetylcholine receptor" Proc. Natl. Acad. Sci., USA 81:155-159 (1984).
Fischbach and Schuetze, "A post-natal decrease in acetylcholine channel open time at rat end-plates" J. Physiol. 303:125-137 (1980).
Giraudat et al., "Structure of the High-Affinity Binding Site for Noncompetitive Blockers of the Acetylcholine Receptor: [.sup.3 H]-Chlorpromazine Labels Homologous Residues in the .beta. and .delta. Chains" Biochem. 26:2410-2418 (1987).
Goldman et al., "Muscle Denervation Increases the Levels of Two mRNAs Coding for the Acetylcholine Receptor .alpha.-Subunit" J. Neurosci. 5:2553-2558 (1985).
Goldman et al., "Mapping of brain areas expressing RNA homologous to two different acetylcholine receptor .alpha.-subunit cDNAs" Proc. Natl. Acad. Sci., USA 83:4076-4080 (1986).
Goldman et al., "Members of a Nicotinic Acetylcholine Receptor Gene Family Are Expressed in Different Regions of the Mammalian Central Nervous System" Cell 48:965-973 (1987).
Gorman et al., "Recombinant Genomes Which Express Chloramphenicol Acetyltransferase in Mammalian Cells" Mol. Cell. Biol. 2(9):1044-1051 (1982).
Greene and Tischler, "Establishment of noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor" Proc. Natl. Acad. Sci., USA 73:(7)2424-2428 (1976).
Grenningloh et al., "The strychnine-binding subunit of the glycine receptor shows homology with nicotinic acetylcholine receptors" Nature 328:215-220 (1987).
Guy, H. R., "A structural model of the acetylcholine receptor channel based on partition energy and helix packing calculations" Biophys. J. 45:249-261 (1984).
Guy and Hucho, "The ion channel of the nicotinic acetylcholine receptor" Trends Neurosci 10(8):318-321 (1987).
Hanke and Breer, "Channel properties of an insect neuronal acetylcholine receptor protein reconstituted in planar lipid bilayers" Nature 321:171-174 (1986).
Hartzell and Fambrough, "Acetylcholine Receptors: Distribution and extrajunctional density in rat diaphragm after denervation correlated with acetylcholine sensitivity" J. Gen. Physiol. 60:248-262 (1972).
Heidmann et al., "Chromosomal Localizaton of Muscle Nicotinic Acetylcholine Receptor Genes in the Mouse" Science 234:866-868 (1986).
Heinemann et al., "Molecular Biology of the Muscle Land Neural Acetylcholine Receptors" In: Nicotinic Acetylcholine Receptor: Structure and Function, NATO ASI Series H, A. Maelicke, ed. (Berlin: Springer-Verlag), H3:359-387 (1986).
Higgins and Berg, "Immunological Identification of a Nicotinic Acetylcholine Receptor on Bovine Chromaffin Cells" J. Neurosci. 7(6):1792-1798 (1987).
Houser et al., "Organization and Morphological Characteristics of Cholinergic Neurons: an Immunocytochemical Study with a Monoclonal Antibody to Choline Acetyltransferase" Brain Res. 266:97-119 (1983).
Hucho et al., "The ion channel of the nicotinic acetylcholine receptor in formed by the homologous helices M II of the receptor subunits" FEBS Lett. 205(1)137-142 (1986).
Huganir et al., "Phosphorylation of the nicotinic acetylcholine receptor regulates its rate of desensitization" Nature 321:774-776 (1986).
Ichikawa and Hirata, "Organization of Choline Acetyltransferase-Containing Structures in the Forebrain of the Rat" J. Neurosci. 6(1):281-292 (1986).
Imoto et al., "Location of a .delta.-subunit region determining ion transport through the acetylcholine receptor channel" Nature 324:670-674 (1986).
Jaynes et al., "Transcriptional Regulation of the Muscle Creatine Kinase Gene and Regulated Expression in Transfected Mouse Myoblasts" Mol. Cell. Biol. 6(8):2855-2864 (1986).
Kao and Karlin, "Acetylcholine Receptor Binding Site Contains a Disulfide Cross-link between Adjacent Half-Cystinyl Residues" J. Biol. Chem. 261(18):8085-8088 (1986).
Karlin, A., "Chemical Modification of the Active Site of Acetylcholine Receptor" J. Gen. Phys. 54:245s-264s (1969).
Kemp et al., "Purification and Characterization of the .alpha.-Bungarotoxin Binding Protein from Rat Brain" Brain Res. 347:274-283 (1985).
Klarsfeld et al., "A 5-Flanking Region of the Chi

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