Neurokinin receptor cell lines

Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Rodent cell – per se

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4353201, 435 691, 4351723, 435354, 435356, 536 2351, 935 66, 935 9, 935 70, C12N 506

Patent

active

058077421

ABSTRACT:
A method for producing high level expression of neuropeptide receptors and stable cell lines useful therein. This method involves culturing a cell line containing amplified copies of a cDNA sequence encoding a neuropeptide receptor containing an expression control sequence taken from the .beta.-actin transcriptional control element.

REFERENCES:
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Letter of Brobbie A. Brandon, Head of the ATCC Patent Depository, dated May 17, 1991.
Henderson, A.K., et al., The Cloned Neurokinin A Receptor Mediates Polyphosphinositide Hydrolysis in Transfected Murine Fibroblasts, FASEB J. 4(3)A315; abstract #288 (1990).
Gunning, P., et al., A Human .beta.-Actin Expression Vector System Directs High-Level Accumulation of Antisense Transcripts, PNAS 84, 4831-4835 (1987).
Masu, Y., et al., cDNA Cloning of Bovine Substance-K Receptor Through Oocyte Expression System, Nature 329, 836-838 (1987).
Couture, R., et al., Characterization of the Peripheral Action of Neurokinins and Neurokinin Receptor Selective Agonist On the Rat Cardiovascular System, Archives of Pharmacology 340, 547-557 (1989).
Hashimoto, T. et al., Synthesis of Neurokinin B Analogs and Their Activities As Agonist and Antagonists, Chem. Phar. Bull. 35(8), 3442-3446 (1987).
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Henderson et al abstract FASEB J 4(3) A315. 1990.
Gunning et al PNAS 84:4831. 1987.
Masu et al Nature 329:836. 1987.
Couture et al NS Arch. Pharmacal 340:547. 1987.
Mashimoto et al Chem. Phar. bull. 35(8):3442. 1987.

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