Neublastin expression constructs

Details Neublastin expression constructs Neublastin expression constructs

2004-10-01

2009-10-06

Saoud, Christine J (Department: 1647)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

C435S069100, C435S325000, C435S358000, C435S320100, C536S023400, C536S023510

Reexamination Certificate

active

07598059

ABSTRACT:
A method of producing a secreted neublastin polypeptide using a heterologous signal sequence is disclosed. The secreted neublastin does not contain a neublastin pro sequence.

REFERENCES:
patent: 6083725 (2000-07-01), Selden et al.
patent: 6361771 (2002-03-01), Tao et al.
patent: 2002/0002269 (2002-01-01), Milbrandt et al.
patent: 2002/0055467 (2002-05-01), Johansen et al.
patent: 2005/0089960 (2005-04-01), Wahlberg et al.
patent: 2007/0238650 (2007-10-01), Sah et al.
patent: WO 93/06116 (1993-04-01), None
patent: WO 97/08196 (1997-03-01), None
patent: WO99/13090 (1999-03-01), None
patent: WO 00/01815 (2000-01-01), None
patent: WO 00/04050 (2000-01-01), None
patent: WO 00/18799 (2000-04-01), None
patent: WO 01/47946 (2001-07-01), None
patent: WO02/46430 (2002-06-01), None
patent: WO 02/051433 (2002-07-01), None
patent: WO 02/060929 (2002-08-01), None
patent: WO 02/072826 (2002-09-01), None
patent: WO 02/078730 (2002-10-01), None
patent: WO 2004/094592 (2004-11-01), None
patent: WO 2004/108760 (2004-12-01), None
patent: WO 2005/039643 (2005-05-01), None
Wang et al. World Journal of Gastroenterology. 8(2): 253-257, 2002.
Andres et al. (2001) “Multiple effects of artemin on sympathetic neurone generation, survival and growth,” Development 128:3685-3695.
Aebischer et al. (1996) “Intrathecal delivery of CNTF using encapsulated genetically modified xenogeneic cells in amyotrophic lateral sclerosis patients,” Nature Medicine, 2:696-699.
Aebischer et al (2001) “Recombinant proteins for neurodegenerative diseases: the delivery issue,” Trends in Neuroscience, Elsevier, Amsterdam, NL 24(9):533-540.
Baloh et al. (1998) “Artemin, a novel member of the GDNF Ligand family, supports peripheral and central neurons and signals through the GFRα3-RET Receptor complex,” Neuron 21:1291-1302.
Baloh et al. (2000) “Functional mapping of receptor specificity domains of glial cell line-derived neurothropic factor (GDNF) family ligands and production of GFR alpha 1 RET-specific agonists,” J. of Biological Chemistry 275(5):3412-3420.
Baudet et al. (2000) “Positive and negative interactions of GDNF, NTN and ART in developing sensory neuron subpopulations, and their collaboration with neurotrophins,” Development 127:4335-4344.
Bauskin et al. (2000) “The propeptide of macrophage inhibitory cytokine (MIC-1), a TGF-β superfamily member, acts as a quality control determinant for correctly folded MIC-1,” The EMBO Journal 19(10):2212-2220.
Bendtsen et al. (2004) “Improved prediction of signal peptides—SignalP 3.0,” J. Mol. Biol. 340(4):783-795.
Bootcov et al. (1997) “MIC-1, a novel macrophage inhibitory cytokine, is a divergent member of the TGF-β superfamily,” PNAS, Immunology, Cell Biology 94:11514-11519.
Enomoto et al. (2001) “RET signaling is essential for migration, axonal growth and axon guidance of developing sympathetic neurons,” Development 128:3963-3974.
Fairlie et al. (2001) “The propeptide of the transforming growth factor-β superfamily member, macrophage inhibitory cytokine-1 (MIC-1), is a multifunctional domain that can facilitate protein folding and secretion,” J. of Biol. Chem. 276(20):16911-16918.
Flanders et al. “TGFβ,” Laboratory of Cell Regulation and Carcinogenesis, National Cancer Institute pp. 719-746.
Griffin et al. (2001) “Assessment of cutaneous innervation by skin biopsies,” Current Opinion in Neurology 14:655-659.
Hoane et al. (2000) “Mammalian-Cell-Produced Neurturin (NTN) Is More Potent Than PurifiedEscherichia coli-Produced NTN,” Exp. Neurol. 162:189-193.
Li et al. (2003)“Expression, purification, and characterization of recombinant human neurturin secreted from the yeastPichia pastoris,” Protein expression and purification 30(1):11-17.
Milbrandt et al. (1998) “Persephin, a novel neurotrophic factor related to GDNF and Neurturin,” Neuron 20:245-253.
Moustakas et al. (2001) “Smad regulation in TGF-β signal transduction,” J. of Cell Science 114:4359-4369.
Nielsen et al. (1997) “Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites,” Protein Engineering 10(1):1-6.
Nielsen et al. (1998) “Prediction of signal peptides and signal anchors by a hidden Markov model,” Proceedings of the 6th International Conference on Intelligent systems for Molecular Biology, pp. 122-130.
Nishino et al. (1999) “GFR alpha3, a component of the artemin receptor, is required for migration and survival of the superior cervical ganglion,” Neuron 23(4):725-736.
Rattenholl et al. (2001) “The pro-sequence facilitates folding of human nerve growth factor fromEscherichia coliinclusion bodies,” Eur. J. Biochem. 268:3296-3303.
Rattenholl et al. (2001) “Pro-sequence assisted folding and disulfide bond formation of human nerve growth factor,” J. Mol. Biol. 305:523-533.
Reinshagen et al. (2000) “Commercial recombinant human β-Nerve Growth factor and adult rat dorsal root ganglia contain an identical molecular species of nerve growth factor prohormone,” J. of Neurochemistry 74:2127-2133.
Rosenblad et al. (2000) “In vivo protection of nigral dopamine neurons by lentiviral gene transfer of the novel GDNF-family member neublastin/artemin,” Molecular and Cellular Neuroscience 15(2):199-214.
Rosenblad et al. (2001) “In vivo protection of nigral dopamine neurons by lentiviral gene transfer of the novel GDNF-family member neublastin/artemin,” Mol. Cell Neurosci. 18(3):332-333.
Saarma et al. (1999) “Other neurotrophic factors: glial cell line-derived neurotrophic factor (GDNF),” Microsc. Res. Tech. 45(4-5):292-302.
Saarma, M. (2000) “GDNF: A stranger in the TGF-beta superfamily?” European Journal of Biochemistry 267(24):6968-6971.
Tseng et al. (1998) “Neurturin protects dopaminergic neurons following medial forebrain bundle axotomy,” Mol. Neurosci 9:1817-1822.
Bonde et al., “GDNF and neublastin protect against NMDA-induced excitotoxicity in hipocampal slice cultures,” Neuroreport 11:4069-4073 (2000).
Bork, “Go hunting in sequence databases but watch out of the traps,” Trends in Genetics 12:425-427 (1996).
Bork, “Powers and Pitfalls in Sequence analysis: the 70% Hurdle,” Genome Research 10:398-400 (2000).
Brenner “Errors in genome annotation,” Trends in Genetics 15:132-133 (1999).
Doerks et al. “Protein annotation: detective work for function prediction,” Trends in Genetics 14:248-250 (1998).
Fjord-Larsen, L. et al. “Efficient in vivo protection of nigral dopaminergic neurons by lentiviral gene transfer of a modified Neurturin construct,” Experimental Neurology, pp. 49-60 (2005).
Merlo et al. “The Mouseint-2Gene Exhibits Basic Fribroblast Growth Facctor Activity in a Basic Fibroblast Growth Factor-responsive Cell Line,” Cell Growth & Differentiation 1:463-472 (1990).
Skolnick et al. “From genes to protein structure and function: novel applications of computational approaches in the genomic era,” Trends in Biotech. 18(1):34-39 (2000).
Smith et al. The challenges of genome sequence annotation or “The devil is in the details,” Nature Biotechnology 15:1222-1223 (1997).
Wells, “Additivity of Mutational Effects in Proteins,” Biochemistry 29:8509-8517 (1990).
Hall, J. et al., “Eukaryotic and Prokaryotic Signal Peptides Direct Secretion of a Bacterial Endoglucanase by Mammalian Cells,” 1990,Journal of Biological Chemistry, 265(32):19996-19999.
Maeda, Y. et al., “Efficient Production of Active TNF α By albumin Signal Peptide,” 1997,Biochemistry and Molecular Biology International, Academic Press, London, GB, 42(4):825-832.
Veronese, F.M. et al., “Introduction and Overview of Peptid

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