Nested PCR employing degradable primers

Details Nested PCR employing degradable primers Nested PCR employing degradable primers

2007-09-25

2007-09-25

Horlick, Kenneth R. (Department: 1637)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S006120

Reexamination Certificate

active

10445097

ABSTRACT:
The invention is directed to one-tube nested nucleic acid amplification methods, as well as compositions for performing these methods, which employ one or more outer primers containing one or more exo-sample nucleotides and inner primers. Nested amplification reactions are performed in these methods in the presence of an agent that degrades the exo-sample-nucleotide-containing primers in time course fashion during the PCR cycles.

REFERENCES:
patent: 4582788 (1986-04-01), Erlich
patent: 4663290 (1987-05-01), Weis et al.
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 4889818 (1989-12-01), Gelfand et al.
patent: 4965188 (1990-10-01), Mullis et al.
patent: 5035996 (1991-07-01), Hartley
patent: 5137814 (1992-08-01), Rashtchian et al.
patent: 5229283 (1993-07-01), Berninger
patent: 5314809 (1994-05-01), Erlich et al.
patent: 5334515 (1994-08-01), Rashtchian et al.
patent: 5340728 (1994-08-01), Grosz et al.
patent: 5348853 (1994-09-01), Wang et al.
patent: 5374553 (1994-12-01), Gelfand et al.
patent: 5418149 (1995-05-01), Gelfand et al.
patent: 5556773 (1996-09-01), Yourno
patent: 5668005 (1997-09-01), Kotewicz et al.
patent: 5683896 (1997-11-01), Hartley et al.
patent: 5853980 (1998-12-01), Rollin et al.
patent: 5866336 (1999-02-01), Nazarenko et al.
patent: 5945313 (1999-08-01), Hartley et al.
patent: 6063608 (2000-05-01), Kotewicz et al.
patent: 6287823 (2001-09-01), Hartley
patent: 0 050 424 (1982-04-01), None
patent: 0 084 796 (1983-08-01), None
patent: 0 201 184 (1986-12-01), None
patent: 0 237 362 (1987-09-01), None
patent: 0 258 017 (1988-03-01), None
patent: WO96/10640 (1996-04-01), None
Akins, R.A., et al., “Mitochondrial Plasmids of Neurospora: Integration into Mitochondrial DNA and Evidence for Reverse Transcription in Mitochondria,”Cell 47:505-516, Cell Press (1986).
Alexander, F., et al., “Proteolytic Processing of Avian Sarcoma and Leukosis Virusespol-endoRecombinant Proteins Reveals AnotherpolGene Domain,”J. Virol. 61:534-542, American Society for Microbiology (1987).
Amersham Pharmacia Biotech, “Drop-In/drop-Out Nested PCR,” 1 page, available at http://autodna.apbiotech.com/handbook/mut/mu4-20.htm, (updated Feb. 7, 2000).
Barr, P.J., et al., “Expression of Active Human Immunodeficiency Virus Reverse Transcriptase inSaccharomyces cerevisiae,” Bio/Tech. 5:486-489, Nature Publishing Company (1987).
Berg, J., et al., “Rapid-Cycle PCR in Temporarily Compartmentalized Capillaries: Two-Round PCR in a Single Capillary Prevents Product Carry-Over,”BioTech. 29:680-683, Eaton Publishing Company (2000).
Berg, J., et al., “Single-tube two-round polymerase chain reaction using the LightCycler™ instrument,”J. Clin: Virol. 20:71-75, Elsevier Science B.V. (Jan. 2001).
Duncan, B.K., “DNA Glycosylases,” in:The Enzymes, Boyer, P.D., ed., Academic Press, New York, NY, pp. 565-586 (1981).
Farmerie, W.G., et al., “Expression and Processing of the AIDS Virus Reverse Transcriptase inEscherichia coli,” Science 236:305-308, American Association for the Advancement of Science (1987).
Fawcett, D.H., et al., “Transposable Elements Controlling I-R Hybrid Dysgenesis in D. melanogaster Are Similar to Mammalian LINEs,”Cell 47:1007-1015, Cell Press (1986).
Gerard, G.F., et al., “Influence on Stability inEscherichia coliof the Carboxy-Terminal Structure of Cloned Moloney Murine Leukemia Virus Reverse Transcriptase,”DNA 5:271-279, Mary Ann Liebert, Inc., Publishers (1986).
Grace, M.B., et al., “Degradable dUMP Outer Primers in Merged Tandem (M/T)-Nested PCR: Low- and Single-Copy DNA Target Amplification,”Anal. Biochem. 263:85-92, Academic Press (1998).
Grandgenett, D.P., et al., “A Single Subunit from Avian Myeloblastosis Virus with Both RNA-Directed DNA Polymerase and Ribonuclease H Activity,”Proc. Natl. Acad. Sci. USA 70:230-234, National Academy of Sciences (1973).
Hansen, J., et al., “RNase H Activity Associated with Bacterially Expressed Reverse Transcriptase of Human T-cell Lymphotropic Virus III/ Lymphadenopathy-associated Virus,”J. Biol. Chem. 262:12393-12396, The American Society for Biochemistry and Molecular Biology (1987).
Holland, P.M., et al., “Detection of specific polymerase chain reaction product by utilizing the 5′→3′ exonuclease activity ofThermus aquaticus,” Proc. Natl. Acad. Sci. USA 88:7276-7280, National Academy of Sciences (1991).
Hu, Y., “A Single Tube Nested PCR for Detection ofShigella spp,” Poster abstractpresented at the 2001 FDA Science Forum, (Jan. 2001).
Kotewicz, M.L., et al., “Cloning and overexpression of Moloney murine leukemia virus reverse transcriptase inEscherichia coli,” Gene 35:249-258, Elsevier Science Publishers (1985).
Krokan, H.E., et al., “DNA glycosylases in the base excision repair of DNA,”Biochem. J. 325:1-16, The Biochemical Society/Portland Press (1997).
Larder, B., et al., “AIDS virus reverse transcriptase defined by high level expression inEscherichia coli,” EMBO J. 6:3133-3137, IRL Press Ltd. (1987).
Lee, L.G., et al., “Allelic discrimination by nick-translation PCR with fluorogenic probes,”Nucl. Acids Res. 21:3761-3766, Oxford University Press (1993).
Life Technologies, “Uracil DNA Glycosylase, ” available online at http://order.lifetech.com/flow.icl?sku=18054015&orderidentifier=ID9924603136564F154, (available Jun. 2001).
Loh, E.Y., et al., “Polymerase Chain Reaction with Single-Sided Specificity: Analysis of T Cell Receptor δ Chain,”Science 243:217-220, American Association for the Advancement of Science (1989).
Michel, F. and Lang, B.F., “Mitochondrial class II introns encode proteins related to the reverse transcriptases of retroviruses,”Nature 316:641-643, Macmillan Publishers (1985).
Mullis, K., et al., “Specific Enzymatic Amplification of DNA In Vitro: The Polymerase Chain Reaction,”Cold Spring Harbor Symp. Quant. Biol. 51:263-273, Cold Spring Harbor Laboratory (1986).
Nazarenko, I.A., et al., “A closed tube format for amplification and detection of DNA based on energy transfer,”Nucl Acids Res. 25:2516-2521, Oxford University Press (1997).
Pecharatana, S., et al., “Genotyping Ocular Strains ofChlamydia trachomatisby Single-tube Nested PCR,”PCR Meth. Appl. 3:200-204, Cold Spring Harbor Laboratory Press (1993).
Pierre, J. and Laval, J., “Specific Nicking of DNA at Apurinic Sites by Peptides Containing Aromatic Residues,”J. Biol. Chem. 256:10217-10220, The American Society of Molecular Biology and Biochemistry (1981).
Rice, N.R., et al., “ThegagandpolGenes of Bovine Leukemia Virus: Nucleotide Sequence and Analysis,”Virol. 142:357-377, Academic Press (1985).
Roche, “Uracil-DNA Glycosylase,” Roche Catalog, Cat. Nos. 1 269 062 and 1 444 646, 2 pages (1999).
Roche, “Uracil-DNA Glycosylase, heat-labile,” Roche Catalog, Cat. Nos. 1 775 367 and 1 775 375, 2 pages (2000).
Roche, “Uracil-DNA Glycosylase,” Roche Catalog, Cat. Nos. 1 269 062 and 1 444 646, 2 pages (Jun. 2001).
Roche, “Uracil-DNA Glycosylase, heat-labile,” Roche Catalog, Cat. Nos. 1 775 367 and 1 775 375, 2 pages (May 2001).
Roth, M.J., et al., “Purification and Characterization of Murine Retroviral Reverse Transcriptase Expressed inEscherichia coli,” J. Biol. Chem. 260:9326-9335, The American Society of Biological Chemists, Inc. (1985).
Saiki, R.K., et al., “Enzymatic Amplification of β-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell Anemia,”Science 230:1350-1354, American Association for the Advancement of Science (1985).
Saiki, R.K., et al., “Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase,”Science 239:487-491, American Association for the Advancement of Science (19

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