Nested oligonucleotides containing a hairpin for nucleic...

Details Nested oligonucleotides containing a hairpin for nucleic... Nested oligonucleotides containing a hairpin for nucleic...

2005-07-19

2005-07-19

Horlick, Kenneth R. (Department: 1637)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S091100, C435S006120, C536S023100, C536S024300, C536S024320, C536S024330

Reexamination Certificate

active

06919189

ABSTRACT:
Templates that are engineered to contain a predetermined sequence and a hairpin structure are provided by a nested oligonucleotide extension reaction. The engineered template allows Single Primer Amplification (SPA) to amplify a target sequence within the engineered template. In particularly useful embodiments, the target sequences from the engineered templates are cloned into expression vehicles to provide a library of polypeptides or proteins, such as, for example, an antibody library.

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