Nerve identification method

Details Nerve identification method Nerve identification method

1983-06-29

1985-11-12

Coven, Edward M.

Surgery

Truss

Pad

435 4, A61N 500

Patent

active

045521450

ABSTRACT:
A method of identifying the matching nerve fascicles in proximal and distal nerve stumps for reanastomosis comprises histochemically making a first visible image of the pattern of carbonic anhydrase emitted by the distal end of the severed nerve or its trimming and a second image of the pattern of carbonic anhydrase emitted by the proximal end of the severed nerve or its trimming, then comparing the two images and thus identifying the fascicles to be joined. A kit for preparing the images is also disclosed.

REFERENCES:
Sunderland, S.: The Anatomical Basis of Nerve Repair, in Nerve Repair and Regeneration, Ed. Jewett, D. L. and H. R. McCarroll, St. Louis, MO, C. V. Mosby, 1980, pp. 14-35.
Hakstian, R. W.: Funicular Orientation by Direct Stimulation: An Aid to Peripheral Nerve Repair, J. Bone Joint Surg., 50A:1178, 1968.
Gruber, H. and Zenker, W.: Acetylcholinesterase: Histochemical Differentiation Between Motor and Sensory Nerve Fibres, Brain Res. 51:207, 1973.
Gruber, H., Freilinger, G., Holle, J. and Mandl, H.: Identification of Motor and Sensory Funiculi in Cut Nerves and Their Selective Reunion, Brit. J. Plastic Surg. 29:70, 1976.
Riley, D. A., Ellis, S. and Bain, J.: Carbonic Anhydrase Histochemistry Reveals Subpopulations of Myelinated Axons in the Dorsal and Ventral Roots of Rat Spinal Nerves, Soc. for Neurosci., Abstr. 7:257, 1981.
Riley, D. A., Ellis, S. and Bain, J.: Carbonic Anhydrase Activity in Skeletal Muscle Fiber Types, Axons, Spindles, and Capillaries of Rat Soleus and Extensor Digitorum Muscles, J. Histochem. Cytochem. 30:1275, 1982.
Wong, V., Barrett, C. P., Donati, E. J., Eng, L. F. and Guth, L.: Carbonic Anhydrase Activity in First-Order Sensory Neurons of the Rat, J. Histochem. Cytochem. 31:293, 1983.
Riley, Danny A. and David H. Lang, 1983, Carbonic Anhydrase Activity of Human Peripheral Nerves, Anat. Rec. 205:162A-163A.
Riley, Danny A. and David H. Lang, 1983, Carbonic Anhydrase Activity of Human Peripheral Nerves, A Possible Histochemical Aid to Nerve Repair, J. Hand Surg. (In press).
Lonnerholm, G. 1980, Carbonic Anhydrase in Rat Liver and Rabbit Skeletal Muscle; Further Evidence for the Specificty of the Histochemical Cobalt-Phosphate Method of Hansson, J. Histochem. Cytochem. 28:427-433.
Millesi, H.: Interfascicular Nerve Repair and Secondary Repair with Nerve Grafts, in Nerve Repair and Regeneration, Ed. Jewett, D. L. and H. R. McCarroll, St. Louis, MO, C. V. Mosby, 1980, pp. 299-319.
Jabaley, M. E., Wallace, W. H. and Heckler, F. R.: Internal Topography of Major Nerves of the Forearm and Hand: A Current View, J. Hand Surg. 5:1, 1980.
Riley, D. A.: Ultrastructural Evidence for Axon Retraction During the Spontaneous Elimination of Polyneuronal Innervation of the Rat Soleus Muscle, J. Neurocytol. 10:425, 1981.

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