Nerve growth factor/receptor complex

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 74, 435 15, 435194, 436536, 530399, G01N 33567

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active

060717092

ABSTRACT:
The present invention relates to a complex comprising nerve growth factor (NGF) and trk proto-oncogene protein and a complex comprising neurotrophic factors, NT-3 or BDNF, and trkB proto-oncogene protein. The present invention also relates to methods for detecting the presence of NGF, NT-3 or BDNF neurotrophic factors and trk and trk-related proto-oncogene receptors. The present invention further relates to methods that may be used in diagnostics and therapeutics for neurodegenerative diseases such as Alzheimer's and Huntington's by detecting complexes comprising NGF or NGF related neurotrophic factors bound to the product of the tyrosine kinase trk or related trk proto-oncogene family member.

REFERENCES:
patent: 5231001 (1993-07-01), Kaplan et al.
patent: 5622862 (1997-04-01), Squinto et al.
Klein et al., Cell 61: 647-656, 1990.
Klein et al., Cell 65: 189-197, 1991.
Soppet et al., Cell 65: 895-903, 1991.
Squinto et al., Cell 65: 885-893, 1991.
Kaplan et al. "Tyrosine Phosphorylation and Kinase Activity of the trk Proto-Oncongene Product Induced by NGF", Nature, 350(6314):158-160, 1991.
Klein et al. "trkB, a Novel Tyrosine Protein Kinase Receptor Expressed During Mouse Neural Development", EMBO Journal, 8(12):3701-3709, 1989.
Klein et al. "Expression of the Tyrosine Kinase Receptor Gene trkB in Confined to the Murine Embryonic . . . ", Development, 109:845-850, 1990.
Berg et al. J. Biol. Chem. 267(1): 13 (1992).
Hefti et al. "Neurotropic Strategies in Neurodegenerative Disease", UCLA Symposium on Advances in Understanding Neurodegenerative Disorders, Jan. 1992.

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