Animal husbandry – Worm culture
Patent
1996-01-29
1997-12-09
Wilson, John J.
Animal husbandry
Worm culture
A01K 6703
Patent
active
056948832
DESCRIPTION:
BRIEF SUMMARY
TECHNICAL FIELD
The present invention relates to a Nematoda cultivating method, and more particularly to a method of cultivating Steinernema kushidai (hereinafter simply called kushidai) in medium.
BACKGROUND ART
Nematodes parasitic on insects, in conditions of contact with host insects, enter the blood, body and cavities of the insects through the mouth, anus, spiracles and the like of the insects, and destroy the immune mechanism of the host insects by releasing symbiont bacteria kept in the body, thereby causing the death of the host insects. This characteristic is utilized in a known method for killing insects harmful to plants such as crops to prevent damage to the plants. Nematodes having such characteristic have heretofore been cultivated in large quantities also.
Incidentally, kushidai relating to the present invention is a relatively new type of Nematoda isolated in 1984 by Kushida et al. of the Forestry Experiment Station in Hamakita, Shizuoka Pref. Said kushidai has a strong insecticidal ability to kill larvae of coliopterous insects such as lamelicorn beetles. This is a peculiar property distinct from the strong insecticidal ability of known nematodes parasitic on insects, such as Steinernema carpocapsae, Steinernema feltiae and Heterorhabditis bacteriphora, to kill mainly lepidopterous insects. Thus, it has been suggested that kushidai has an insecticidal mechanism different from other nematodes. It is thought that conditions suited to its cultivation are different from the cultivating technique for other known nematodes parasitic on insects.
The present invention has been made on the finding of the fact that, when a medium containing asteroid is used in cultivating kushidai in medium, said kushidai exhibits a high degree of insecticidal activity. Its object is to provide a method of cultivating large quantities of kushidai having a high degree of insecticidal activity.
DISCLOSURE OF THE INVENTION
To fulfill this object, a first characterizing feature lies in cultivation of kushidai in a medium including asteroid.
Inventors have established, by experiment, the fact that, when cultivating known nematodes parasitic on insects, such as Steinernema carpocapsae strain ALL (hereinafter simply called ALL), cultivation in a medium including a steroid does not increase its insecticidal activity, but cultivation of kushidai in a medium including asteroid may significantly increase its insecticidal activity compared with cultivation in an ordinary medium not including asteroid. Thus, it has been found that, by including asteroid in a kushidai cultivating medium, the steroid specifically acts on kushidai to produce an insecticidal activity increasing effect not seen in other nematodes, and the kushidai cultivated in that medium exhibits a high degree of insecticidal activity.
That is, kushidai having a high insecticidal activity are now obtained by cultivating kushidai in a medium including asteroid.
A second characterizing feature lies in causing a medium (3) including a steroid to be adsorbed to a plurality of supports, then stacking the supports with gaps thereamong to form a layer, and cultivating Steinernema kushidai in said layer.
With such characterizing feature, said gaps allow a sufficient supply of oxygen for cultivating kushidai efficiently.
A third characterizing feature lies in preparing a liquid medium including a steroid, placing the liquid medium in a cultivating container in a condition to supply oxygen into said liquid medium and to agitate the same, and cultivating kushidai in said cultivating container.
With such characterizing feature, since the medium including asteroid is a liquid medium, cultivation may be carried out along with agitation and oxygen supply to cultivate kushidai efficiently.
Said medium including asteroid may be formed by preparing a solution of the steroid dissolved in heated oil and adding the solution to the medium. Said medium including asteroid may be formed by preparing a solution of the steroid dissolved in an organic solvent, adding oil to the solutio
REFERENCES:
patent: 5023183 (1991-06-01), Friedman et al.
Kawasugi Tadaaki
Suzuki Nobukazu
Tachibana Mineo
Uechi Toshihito
Abbott Yvonne R.
Kubota Corporation
Wilson John J.
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