Neisseria gonorrhoeae-specific oligonucleotides

Details Neisseria gonorrhoeae-specific oligonucleotides Neisseria gonorrhoeae-specific oligonucleotides

1998-04-09

2000-07-18

Fredman, Jeffrey

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 5, 435 911, 435 912, 435810, 536 231, 536 243, 536 2431, 536 2432, 536 2433, C12Q 168, C12P 1934, C07H 2102, C07H 2104

Patent

active

060905573

ABSTRACT:
The present invention relates to oligonucleotides which hybridize specifically to the cytosine DNA methyltransferase gene of Neisseria gonorrhoeae and distinguish the cytosine DNA methyltransferase gene of N. gonorrhoeae from highly homologous sequences which have been discovered in some strains of other species of the genus Neisseria. The oligonucleotides are useful as primers for the polymerase chain reaction (PCR) amplification of a nucleic acid sequence from the cytosine DNA methyltransferase gene of N. gonorrhoeae and in N. gonorrhoeae amplification/detection assays.

REFERENCES:
patent: 5256536 (1993-10-01), Miyada et al.
patent: 5525717 (1996-06-01), Miyada et al.
patent: 5550040 (1996-08-01), Purohit et al.
Miyada et al, "A DNA sequence for the discrimination of Neisseria gonorrheae from other Neisseria species", Mol. Cell. Probes 5:327-335, 1991.
1996, Roche Diagnostics Systems, Inc. Amplicor.RTM. product insert for "Chlamydia trachomatis/Neisseria gonorrhoeae (CT/NG) test."

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