Neisseria gonorrhoeae detection

Details Neisseria gonorrhoeae detection Neisseria gonorrhoeae detection

2011-08-16

2011-08-16

Zeman, Robert A (Department: 1645)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C536S023100, C536S024300, C536S024330

Reexamination Certificate

active

07998668

ABSTRACT:
A method for determining whether a human individual is or has been infected withNeisseria gonorrhoeae, is provided. The method detects aNeisseria gonorrhoeae, porA nucleic acid fragment obtained from a biological sample. The method includes subjecting the biological sample to nucleic acid sequence amplification using primers having respective nucleotide sequences according to SEQ ID NO:1 and SEQ ID NO:2, to thereby produce a porANeisseria gonorrhoeae, amplification product. The amplification product is detected by fluorescence resonance energy transfer using oligonucleotides having respective nucleotide sequences according to SEQ ID NO:3 which has a donor fluorophore and SEQ ID NO:4, which has an acceptor fluorophore.

REFERENCES:
Glustein et al., Molecular Diagnosis 4(3): 233-239 (1999).
McKnew et al., Journal of Infectious Diseases 187: 1213-1222 (2003).
Tabrizi et al., Sexually Transmitted Infections 80: 68-71 (2004).
Whiley el al., European Journal of Clinical Microbial Infectious Diseases 23: 705-710 (2004).

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