Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1989-09-13
1992-02-18
Kepplinger, Esther L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 736, 435 72, 435 14, 435 24, 435810, 435871, 435975, 436827, C12Q 104, C12Q 154, C12Q 137, G01N 33571
Patent
active
050893947
ABSTRACT:
A specimen containing N. lactamica, N. meningitidis, N. gonorrhoeae or B. catarrhalis is incubated simultaneously with a first substrate for beta-galactosidase and a second substrate for gamma-glutamyl aminopeptidase to form reaction products with one or the other Neisseriae which yields a detectible first or second signal distinct from each other depending upon the presence of the enzyme. Signals may comprise distinct colors which emit either in the absence or presence of diazo dye coupler. A third substrate specific for prolyliminopeptidase in N. gonorrhoeae may be added to form a detectible third signal of the same type. The third substrate may be incubated simultaneously with the first two substrates or later. The absence of all of the first three signals is a positive indication that the specimen contains B. catarrhalis. Also, a diagnostic test kit for performing the above tests with or without lectin or antibody for agglutination based upon specific recognition.
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Chu Albert E.
Chun Peter K.
E-Y Laboratories, Inc.
Kepplinger Esther L.
Scheiner Toni
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