Negative control probes that are identical in sequence but oppos

Details Negative control probes that are identical in sequence but oppos Negative control probes that are identical in sequence but oppos

1994-04-08

1996-10-08

Arthur, Lisa B.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

536 243, 935 77, 935 78, C12Q 168, C07H 2104

Patent

active

055630340

ABSTRACT:
Two basic design criteria for producing oligonucleotide "negative control probes" for use in nucleic acid hybridization assays are described. Each type of negative control probe also functions as a synthetic polynucleotide "target" that is useful for testing the other type of negative control probe. These design criteria also provide structures that are not naturally occurring, but are analogous to naturally occurring nucleic acids in primary, secondary and tertiary structure. These design criteria are useful in hybridization assays and potentially in other applications as well.

REFERENCES:
patent: 5434047 (1995-07-01), Arnold, Jr.
Giovannoni et al. (Feb. 1988) J. Bacteriol. 170(2): 720-726.

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