Multicellular living organisms and unmodified parts thereof and – Nonhuman animal – Transgenic nonhuman animal
Reexamination Certificate
2005-08-23
2005-08-23
Waitach, Joe (Department: 1632)
Multicellular living organisms and unmodified parts thereof and
Nonhuman animal
Transgenic nonhuman animal
C800S003000, C800S008000, C800S013000, C800S021000, C800S022000, C435S455000, C435S463000, C435S320100, C435S325000
Reexamination Certificate
active
06933422
ABSTRACT:
An object of the present invention is to provide a null mutant non-human animal showing salt intake behavior similar to that of wild-type animals under water-sufficient conditions and showing much more intakes of hypertonic saline compared with wild-type animals under water- and salt-depleted conditions, for example, an Nav2 gene-deficient non-human animal, which is useful as a model animal of excessive salt intake experiments.The object will be attained by following process: mouse genomic libraries are screened with rat NaG cDNA as a probe, then Nav2 gene of genomic DNA is isolated, and a targeting vector is constructed by inserting marker gene such as neo gene into the exon of Nav2. After thus constructed targeting vector is induced to ES cells, homologously recombined ES cells are selected, then germ line chimeric mice are constructed with this ES cells strain, and they are hybridized with the wild-type mice and heterozygous mutant mice are obtained. By intercrossing of thus obtained heterozygous mutant mice, Nav2 knockout mice are constructed.
REFERENCES:
patent: 4946778 (1990-08-01), Ladner
Moreadith, R.W. et al. Gene Targeting in Embryonic Stem Cells: The New Physiology and Metabolism. J. Mol. Med, 1997, 75:208-216.
Sanford et al. Meth in Mol. Bio, 158:217-225, 2001.
Mullins et al. Journal of Clinical Investigation, 1996.
Pera et al. Journal of Cell Science 113: 5-10 (2000).
Encyclopedia Britannica, http://www.search.eb.com/dictionary.
Noda et al, “Existence of Distinct Sodium Channel Messenger RNAs in Rat Brain”, Nature, vol. 320:188-192, (1986).
Kayano et al., “Primary Structure Of Rat Brain Sodium Channel III Deduced From The cDNA Sequence”, Federation of European Biochemical Societies, vol. 228(1):187-194, (1988).
Sontheimer et al., “Voltage-Gated Na+ Channels In Glia: Properties And Possible Functions”, TINS, Elsevier Science Ltd., vol. 19(8):325-331, (1996).
Reese et al., “Immunocytochemical Localization of NaCh6 In Cultured Spinal Cord Astrocytes”, GLIA, Wiley-Liss, Inc., vol. 26:92-96, (1999).
Gautron et al., “The glial Voltage-Gated Sodium Channel: Cell- And Tissue-Specific MRNA Expression”, Proc. Natl. Acad. Sci., USA, Neurobiology, vol. 89:7272-7276, (1992).
George, Jr. et al., “Molecular Cloning Of An Atypical Voltage-Gated Socium Channel Expressed In Human Heart And Uterus: Evidence For A Distinct Gene Family”, Proc. Natl. Acad. Sci, USA, Physiology, vol. 89:4893-4897, (1992).
Felts et al., “NaG: A Sodium Channel-Like mRNA Shared By Schwann Cells And Other Natural Crest Derivatives”, GLIA, Wiley-Liss, Inc., vol. 21:269-276, (1997).
Felts et al., “Sodium Channel a-Subunit mRNAs I, II, III, NaG, Na6 And hNE (Pn1): Different Expression Patterns In Developing Rat Nervous System”, Molecular Brain Research, Elsevier Science B.V., vol. 45:71-82, (1997).
“Continuous Cultures Of Fused Cells Secreting Antibody Of Predefined Specificity”, Nature, vol. 256:495-497, (1975).
Kozbor et al., “The Production Of Monoclonal Antibodies From Human Lymphocytes”, Immunology Today, Elsevier Biomedical Press, vol. 4(3):72-79, (1983).
Cole et al., “The EBV-Hybridoma Technique And Its Application To Human Lung Cancer”, Monoclonal Antibodies And Cancer Therapy, Department of Microbiology & Immunology Queen's University, Alan R. Liss, Inc., pp. 77-96, (1985).
Pietri-Rouxel et al., “The Biochemical Effect Of The Naturally Occuring Trp64→Arg Mutation On Human β3-Adrenoceptor Activity”, Eur. J. Biochem., FEBS, vol. 247:1174-1179, (1997).
Yagi, et al., “A Novel Negative Selection For Homologous Recombinants Using Diphtheria Toxin A Fragment Gene”, Analytical Biochemistry, Academic Press, Inc., vol. 214:77-86, (1993).
Shintani et al., “Neurons As Well As Astrocytes Express Proteoglycan-Type Protein Tyrosine Phosphatase ζ/RPTPβ: Analysis of Mice In Which The PTPζ/RPTPβ Gene Was Replaced With The LacZ Gene”, Neuroscience Letters, Elsevier Science Ireland Ltd., vol. 247:135-138, (1998).
Knittle et al., “Immunolocalization Of The Mnav2.3 Na+ Channel In Mouse Heart: Upregulation In Myometrium During Pregnancy”, The American Physiological Society, pp. C688-C696, (1996).
CH. Pilgrim, “Transport Function Of Hypothalamic Tanycyt Ependyma: How Good Is The Evidence”, Neuroscience, vol. 3:277-283, (1978).
Hatton, “Pituicytes, Glia And Control Of Terminal Secretion”, J. exp. Biol., vol. 139:67-79, (1988).
Bourque et al., “Osmoreceptors In The Central Nervous System”, Annu. Rev. Physiol., vol. 59:601-619, (1997).
Fitzsimons, “Angiotensin, Thirst, And Sodium Appetite”, Physiological Reviews, vol. 78(3):583-686, (1998).
Andersson, “Regulation of Water Intake”, Physiological Reviews, vol. 58(3):582-603, (1978).
Johnson et al., “The Extended Amygdala And Salt Appetite”, Annals New York Academy of Sciences, vol. 877:258-280, (1999).
Davis et al., Basic Methods in Molecular biology, (1986).
Joseph Sambrook et al..Molecular Cloning: A Laboratory Manual, 1989, 3rdEd., vols. 1-3, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
M. Renganathan et al. “α-SNS Produces, the Slow TTX-Resistant Sodium Current in Large Cutaneous Afferent DRG Neurons”.J. Neurophysiol, 2000, pp. 710-718, vol. 84.
Mirek Jurzak et al., “Primary Culture Of Circumventricular Organs From the Rat Brain Lamina Terminals”,Brain Research, 1994, vol. 662, pp. 198-208.
Alan Kim Johnson, “Sensory Circumventricular Organs and Brain Homeostatic Pathways”, Federation of American Societies for Experimental Biology, vol. 7, No. 8, 1993, pp. 678-686.
Antonio Felipe et al., “Primary Structure and Differential Expression During Development and Pregnancy of a Novel Voltage-gated Sodium Channel in the Mouse”, Journal of Biological Chemistry, vol. 269, No. 48, 1994, pp. 30125-30131.
A. N. Akopian et al, “Structure and Distributiono f a Broadly Espressed Atypical Sodium Channel”, Febs Letters, Elsevier Science Publishers, Amsterdam, NL, vol. 400, No. 2, Jan. 3, 1997, pp. 183-187.
Stephen J. Quinn et al, “Sodium and Ionic Strenght Sensing by the Calcium Receptor”, Journal of Biological Chemistry, vol. 273, No. 31, Jul. 31, 1998, pp. 19579-19586.
Eiji Watanabe et al., “Nav2/NaG Channel is Involved in Control of Salt-intake Behavior in the CNS”, Journal of Neuroscience, vol. 20, No. 20, Oct. 15, 2000, pp. 7743-7751.
Noda Masaharu
Watanabe Eiji
Hobbs Ann S.
Japan as represented by Director of General of Okazaki National
Kinberg Robert
Ton Thai-an N.
Venable LLP
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