Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals – Carrier is particulate and the particles are of...
Reexamination Certificate
2004-07-22
2009-10-06
Gabel, Gailene R (Department: 1641)
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
Carrier is particulate and the particles are of...
C435S002000, C435S006120, C435S007100, C435S007500, C435S007920, C435S007940, C435S173300, C435S287200, C435S374000, C436S506000, C436S507000, C436S524000, C436S528000, C436S546000, C436S177000, C422S082050, C422S082080
Reexamination Certificate
active
07598093
ABSTRACT:
Embodiments of the present invention relate to devices and methods for detecting cellular products using detection particles having product-specific detection reagents and having a characteristic spectral feature. In particular, devices and methods are provided for measuring secreted cellular products including cytokines. Detection substrates, include microwells having product-specific capture reagents thereon or comprising hydrophobic membranes are described having greater capability to detect products from individual cells in a mixture of heterogeneous cells. With the use of multiple detection particles, multiple cellular products can be detected in a single well. Additionally, using the inherent spectral properties of detection particles, no enzymatic reactions are needed to visualize a secreted product, thereby increasing the sensitivity, reproducibility and ease of use.
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Karulin Alexey Y.
Kleen Thomas Oliver
Lehmann Paul V.
Borson D. Benjamin
CTL Analyzers, LLC
Gabel Gailene R
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