N-terminal sequencing of proteins and peptides

Details N-terminal sequencing of proteins and peptides N-terminal sequencing of proteins and peptides

1993-09-15

1995-07-11

Warden, Jill

Chemistry: analytical and immunological testing

Peptide, protein or amino acid

Glycoproteins

436 89, 436 90, 530345, 530408, A61K 3800, C07K 100, C07K 1700

Patent

active

054320917

ABSTRACT:
An improved method for the N-terminal sequential degradation of proteins and peptides is disclosed. The protein or peptide to be sequenced is reacted with a compound effective to impart a tertiary amine functionality to the thiazolinone derivative of a cleaved terminal amino acid of the peptide.

REFERENCES:
patent: 4861726 (1989-08-01), Stolowitz et al.
patent: 5240859 (1993-08-01), Aebersold
Zubay "Biochemistry", 2nd ed. MacMillan Publishing Co. 1988, p. 48.
Salnikow, et al., Methods in Protein Sequence Analysis (Ed. Elzinga, M.) Humana Press, Clifton, N.J., pp. 181-188 (1982).
Jin, S. W., et al., FEBS Lett. 198:34-41 (1989).
Horn, M. J., Techniques in Protein Chemistry (Ed., Hugli, E. T.) Academic Press, San Diego, pp. 51-58 (1989).
Margolies, M. N., et al., Methods in Protein Sequence Analysis (Ed. Elzinga, M.) Humana Press, Clifton, N.J. pp. 189-203 (1982).
Jin et al. FEBS Lett. vol. 198 (1986) p. 150.

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