N-terminal and C-terminal markers in nascent proteins

Details N-terminal and C-terminal markers in nascent proteins N-terminal and C-terminal markers in nascent proteins

2004-11-19

2008-09-09

Ketter, James S (Department: 1636)

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

C530S388100

Reexamination Certificate

active

07423122

ABSTRACT:
This invention relates to non-radioactive markers that facilitate the detection and analysis of nascent proteins translated within cellular or cell-free translation systems. Nascent proteins containing these markers can be rapidly and efficiently detected, isolated and analyzed without the handling and disposal problems associated with radioactive reagents. Methods are described for incorporating N-terminal, C-terminal and (optionally) affinity markers into a nascent protein

REFERENCES:
patent: 5643722 (1997-07-01), Rothschild et al.

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