N.sup.4 -methyl-2'-deoxycytidine 5'-triphosphate and its use in

Details N.sup.4 -methyl-2'-deoxycytidine 5'-triphosphate and its use in N.sup.4 -methyl-2'-deoxycytidine 5'-triphosphate and its use in

1992-09-04

1994-05-31

Parr, Margaret

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, 536 785, 536 7851, 536 784, C12P 1934, C07H 1906, C07H 19073

Patent

active

053169484

ABSTRACT:
The present invention provides novel compounds, N.sup.4 -methylcytidine 5'-triphosphate (N.sup.4 -methyl-CTP) and N.sup.4 -methyl-2'-deoxycytidine 5'-triphosphate (N.sup.4 -methyl-dCTP). These compounds serve as substrates for RNA or DNA polymerases for incorporation of the N.sup.4 -methyl-C moiety into oligo- and polynucleotides. This is advantageous for certain purposes. For example, DNA sequence analysis using the standard dideoxy chain termination method can be carried out using N.sup.4 -methyl-dCTP in the reaction mixture instead of dCTP. In the gel electrophoresis separation of the resulting oligonucleotides, artifacts of band compression and band distention are eliminated. The novel compounds may also be useful in other applications of primer extension reactions, for example, nucleic acid amplification schemes that employ transcription reactions or RNA replicases.

REFERENCES:
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