Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1992-04-24
1995-06-27
Rollins, John W.
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
536 2851, 536 253, 536 2531, C07H 19073
Patent
active
054281482
ABSTRACT:
Disclosed herein are protecting groups for exocyclic amino groups of the bases adenine, guanine and cytosine for use in the synthesis of oligonucleotides, the protecting groups being represented by the formula: --CO-- (CH.sub.2).sub.0-9 --CH.sub.3. In a particularly preferred embodiment, the base cytosine is protected with acetyl (--CO--CH.sub.3), and the oligonucleotide incorporating said protected cytosine is subjected to a cleavage/deprotection reagent comprising at least one straight chain alkylamine having from 1 to about 10 carbon atoms.
REFERENCES:
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Chemical Abstracts "File CA" structure searches, completed Mar. 23, 1993.
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Vu, H., et al "Fast Oligonucleotide Deprotection Phosphoramidite Chemistry for DNA Synthesis" Tetrahedron Letters 31(50): 7269-7272 (1990).
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Hanna Naeem B.
Reddy Parameswara M.
Beckman Instruments Inc.
Crane L. Eric
Harder P. R.
Henry Janis C.
May William H.
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