N-succinimidyl haloacetyl aminobenzoates as coupling agents

Details N-succinimidyl haloacetyl aminobenzoates as coupling agents N-succinimidyl haloacetyl aminobenzoates as coupling agents

1979-11-13

1981-02-17

Daus, Donald G.

Chemistry of carbon compounds

Miscellaneous organic carbon compounds

C-metal

C07D20746

Patent

active

042514452

ABSTRACT:
Novel enzyme conjugates useful in immunoassay methods are prepared with the use of a novel coupling reagent of N-succinimidyl (4-iodoacetyl) aminobenzoate by reacting the coupling reagent, firstly, with an amino-containing macromolecule, and, thereafter, with a sulfhydryl-containing enzyme, the enzyme conjugate prepared in a high yield and of high specificity.

REFERENCES:
patent: 3541084 (1970-11-01), Hagitani et al.
W. Higgins et al., Chem. Abstracts 89: 142590d, (1978).
D. Santi et al., Chem. Abstracts 83: 175111c, (1975).
T. Kitagawa et al., J. Biochem., 79, 233-236, (1976). Enzyme Coupled Immunoassay of Insulin Using a Novel Coupling Reagent.

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