N protein mutants of porcine reproductive and respiratory...

Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Virus or component thereof

Reexamination Certificate

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C536S023720, C435S235100, C435S236000, C514S04400A

Reexamination Certificate

active

07544362

ABSTRACT:
The present invention provides a genetically modified PRRS virus, methods to make it and related polypeptides, polynucleotides and various components. Vaccines comprising the genetically modified virus and polynucleotides are also provided.

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Yang et al (Archives of Virology 144:525-546, 1999).
Shen et al (Archives of Virology 145:871-883, 2000)].
NCBI AF184212, Sep. 28, 2000.
Le Gall et al (Virus Research 54:9-21, 1998).
Lee, C., Calvert, J. G., Welch, S.-K. W., and Yoo, D. (2005). A DNA-launched reverse genetics system for porcine reproductive and respiratory syndrome virus reveals that homodimerization of the nucleocapsid protein is essential for virus infectivity. Virology 331, 47-62.
Rowland, R. R., Kervin, R., Kuckleburg, C., Sperlich, A., and Benfield, D. A. (1999). The localization of porcine reproductive and respiratory syndrome virus nucleocapsid protein to the nucleolus of infected cells and identification of a potential nucleolar localization signal, Virology 316, 135-145.
Rowland, R. R. R., Schneider, P., Fang, Y., Wootton, S., Yoo, D., and Benfield, D. A. (2003). Peptide domains involved in the localization of the porcine reproductive and respiratory syndrome virus nucleocapsid protein to the nucleolus. Virology 316(1), 135-145 (Abstract).
Rowland, R. R. R., and Yoo, D. (2003). Nucleolar-cytoplasmic shuttling of PRRSV nucleocapsid protein: a simple case of molecular mimicry or the complex regulation by nuclear import, nucleolar localization and nuclear export signal sequences. Virus Research 95, 23-33.
Wootton, S. K., Rowland, R. R. R., and Yoo, D. (2002). Phosphorylation of the porcine reproductive and respiratory syndrome virus nucleocapsid protein. Journal of Virology 76 (20), 10569-10576.
Wootton, S. K., and Yoo, D. (2003). Homo-oligomerization of the porcine reproductive and respiratory syndrome virus nucleocapsid protein and the role of disulfide linkages. Journal of Virology 77(8), 4546-4557.
Yoo, D., Wootton, S. K., Li, G., Song, C., and Rowland, R. R. (2003). Colocalization and interaction of the porcine arterivirus nucleocapsid protein with the small nucleolar RNA-associated protein fibrillarin. Journal of Virology 77(22), 12173-12183.
Yoo, D., Welch, S.-K. W., Lee, C., and Calvert, J. G. (2004). Infectious clones of porcine reproductive and respiratory syndrome virus and their potential as vaccine vectors. Veterinary Immunology and Immunopathology 102, 143-154.

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