N-acylphosphoramidites and their use in oligonucleotide...

Details N-acylphosphoramidites and their use in oligonucleotide... N-acylphosphoramidites and their use in oligonucleotide...

2005-11-15

2005-11-15

Solola, Taofiq (Department: 1626)

Organic compounds -- part of the class 532-570 series

Organic compounds

Heterocyclic carbon compounds containing a hetero ring...

C536S025300, C536S026110

Reexamination Certificate

active

06965041

ABSTRACT:
The present invention provides a compound of formula (I), (II), or (III), wherein R1, R2, R2′, R3, and R3′are the same or different and each is H an alkyl, an alkenyl, an alkynyl, a cycloalkyl, an aryl, or an aralkyl. Alternatively, either of R2or R2′combined with either of R3or R3′comprises a ring. R4is a protecting group or a solid support R5is H or an alkyl. R6is a protecting group, an amidoalkyl, an alkyl, an alkyl ketone, an alkenyl, an alkynyl, a cycloalkyl, an aryl, or an aralkyl. R15is H or a protecting group. Q and Q1are the same or different and each is a nucleoside, an oligonucleotide comprising a nucleoside, or an oligomer comprising a nucleoside, which is of formula (a) or (b), wherein B is a labeling group, an alkyl, an alkenyl, an alkynyl, a cyclic group optionally containing one or more heteroatoms, or an amino; and, E is H, a halogen, a hydroxy, an alkoxy, an ester, an amino or a protecting group. X and X1are independently O, S, or Se, and n is an integer from 1 to about 300. Each Q in each monomeric unit defined by n can be the same or different. The present invention further provides a method of preparing a polymer using the N-acylphosphoramidite of formula (I) or (II).

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