N-acetylmannosamine dehydrogenase, process for its production, m

Details N-acetylmannosamine dehydrogenase, process for its production, m N-acetylmannosamine dehydrogenase, process for its production, m

1990-02-27

1991-08-06

Kepplinger, Esther L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 25, 435 4, 435 18, 435810, C12Q 132, C12Q 126, C12Q 100

Patent

active

050377396

ABSTRACT:
N-Acetylmannosamine dehydrogenase which takes off hydrogen from N-acetylmannosamine to convert it into N-acetylmannosaminolactone and, at the same time, reduces coenzyme NAD into NADH. This enzyme can be obtained by culturing, in a medium, a strain belonging to Genus Flavobacterium and having an ability to produce N-acetylmannosamine dehydrogenase producing activity, and then collecting it. This enzyme is usable in the quantitative analysis of N-acetylmannosamine or sialic acid.

REFERENCES:
Methods in Enzymology, (1963), vol. VI, pp. 465-467.
Clinica Chemica Acta, International Journal of Clinical Chemistry, vol. 108/1 (1980).
Yoneyama et al., "Distribution of Mannosamine and Mannosaminuronic Acid Among Cell Walls of Bacillus Species", J. Bacteriol., vol. 149, No. 1, pp. 15-21, 1982.
Methods of Enzymatic Analysis, Weinheim, vol. VI, Verlg Chemie, pp. 80-90, (1984).

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