N-acetylhexosamine oxidase and process for producing the same

Details N-acetylhexosamine oxidase and process for producing the same N-acetylhexosamine oxidase and process for producing the same

1984-02-15

1986-11-04

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

435874, C12N 906, C12R 138

Patent

active

046210571

ABSTRACT:
An N-acetylhexosamine oxidase having the following physiochemical properties.
(1) Action and specificity for substrate
Oxidizes N-acetylhexosamine in the presence of oxygen to form N-acetylhexosaminic acid and hydrogen peroxide. Hardly acts or does not act at all on hexose and hexosamine.
(2) Optimum pH and stable pH range
When a potassium phosphate buffer solution contining 0.1M glycine is used, an optimum pH is 7.5 to 8.5 and a stable pH range is 3 to 9.
(3) Molecular weight
Has a molecular weight of about 140,000 to 150,000 when measured according to a gel filtration method using Sephadex G-200 by the use of 0.05M potassium phosphate buffer solution.

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