N-acetylhexosamine-dehydrogenase, process for producing same, me

Details N-acetylhexosamine-dehydrogenase, process for producing same, me N-acetylhexosamine-dehydrogenase, process for producing same, me

1989-09-14

1991-11-26

Wax, Robert A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

435183, 435 26, 435810, 435262, 435875, 435 79, 435 791, 435 795, C12N 904, C12Q 132

Patent

active

050681907

ABSTRACT:
N-Acetylhexosamine-dehydrogenase which takes off hydrogen from N-acetylglucosamine or N-acetylgalactosamine to convert them to N-acetylglucosaminolactone or N-acetylgalactosaminolactone, respectively, and, at the same time, reduces co-enzymes NAD.sup.+ to NADH is provided herein.
The enzyme of this invention can be obtained by culturing, in a medium, a strain belonging to Genus Pseudomonas and having an ability to produce N-acetylhexosamine-dehydrogenase, followed by collecting the enzyme from the cultured product.
Herein is also provided a method for quantitatively analyzing N-acetylglucosamine or N-acetylgalactosamine which comprises reacting N-acetylglucosamine-dehydrogenase upon a sample containing N-acetylglucosamine or N-acetylgalactosamine and measuring the quantity of the resulting NADH.

REFERENCES:
patent: 4634663 (1987-01-01), Horiuchi
patent: 4960701 (1990-10-01), Horiuchi et al.
Chemical Abstracts, 101:22633g, 405 (1984).
Chemical Abstracts, 102:60787W, 481 (1985).
Horiuchi et al., "Purification and Characterization of N-Acetyl-D-Hexosamine Dehydrogenase from Pseudomonas sp. No. 53", Agric. Biol. Chem. 53, 1919-25 (1989).

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