Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase
Patent
1996-04-23
1999-09-07
Marx, Irene
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Oxidoreductase
435189, 435 712, 424 944, C12N 908
Patent
active
059486619
DESCRIPTION:
BRIEF SUMMARY
FIELD OF INVENTION
The present invention relates to a novel peroxidase, a process for its production and to its use in bleaching and detergent compositions.
BACKGROUND OF THE INVENTION
Peroxidases (E.C. 1.11.1.7) are enzymes that catalyze the oxidation of a substrate (an electron or hydrogen donor) with hydrogen peroxide. Such enzymes are known from microbial, plant and animal origins, e.g. peroxidase from Coprinus cinereus (cf. e.g. EP 179,486). They are typically hemoproteins, i.e. they contain a heme as a prosthetic group.
It has been found that peroxidases, utilizing hydrogen peroxide as substrate, are able to enhance the bleaching effect of hydrogen peroxide during washing. The use of peroxidases for bleaching stains on fabrics is described in WO 89/09813. It has also been found that coloured substances leached from dyed fabrics could be bleached by means of peroxidases together with hydrogen peroxide. The use of peroxidases for inhibiting dye transfer in this way is described in WO 91/05839.
Certain oxidizable substrates, e.g. metal ions and phenolic compounds such as 7-hydroxycoumarin (7HCm), vanillin (VAN), and p-hydroxybenzenesulfonate (pHBS), have been described as accelerators or enhancers, able to enhance bleaching reactions (cf. e.g. WO 92/18683, WO 92/18687, and Kato M and Shimizu S, Plant Cell Physiol. 1985 26 (7), pp. 1291-1301 (cf. Table 1 in particular). In WO 94/12621 other types of enhancing agents are disclosed, e.g. phenothiazines and phenoxazines.
It is the object of this invention to provide a peroxidase with improved bleaching performance at high pH.
SUMMARY OF THE INVENTION
We have unexpectedly found that a novel peroxidase can be obtained from Myxococcaceae, a family not previously reported to produce peroxidase, and we have further found that, surprisingly, this novel peroxidase shows excellent performance in bleaching assays at pH 10.5.
Accordingly, the invention provides a peroxidase preparation, characterized by being producible by cultivation of a peroxidase producing strain of the family Myxococcaceae.
The invention further provides a process for the production of peroxidase, characterized by comprising cultivation in a suitable nutrient medium of a peroxidase-producing strain of Myxococcaceae. Finally, the invention provides use of said peroxidase in bleaching and detergent compositions.
BRIEF DESCRIPTION OF DRAWINGS
The present invention is further illustrated by reference to the accompanying drawings, in which:
FIG. 1 shows the initial bleaching of Direct Blue 1 (DB1) under the following conditions: 100 .mu.M H.sub.2 O.sub.2 ; 20 mM acetate/phosphate-buffer; 0.12 PODU/ml Myxococcus virescens peroxidase; pH 5 (.quadrature.)-pH 6 (.diamond-solid.)-pH 7 (.box-solid.)-pH 8 (.smallcircle.)-pH 9 (X), respectively; room temperature.
FIG. 2 shows the initial bleaching of DB1 using the conditions just described, but additionally containing 10 .mu.M 10-propionic acid phenothiazine.
FIG. 3 shows the initial bleaching of DB1 at pH 10.5 under the following conditions: 100 .mu.M H.sub.2 O.sub.2 ; 25 mM Borate; 0.5 PODU/ml Myxococcus virescens peroxidase; 0 .mu.M 10-propionic acid phenothiazine (.quadrature.)/10 .mu.M 10-propionic acid phenothiazine (.diamond-solid.), respectively; room temperature.
DETAILED DISCLOSURE OF THE INVENTION
According to the invention, peroxidase is derived from a peroxidase-producing strain of the Myxococcaceae. In the family Myxococcaceae the genera Myxococcus and Corallococcus are preferred, in particular Myxococcus virescens, Myxococcus fulvus, Myxococcus xanthus, Corallococcus coralloides and Corallococcus exiguus.
The family Myxococcaceae is described in Myxobacteria II (M. Dworkin and D. Kaiser, Eds.), American Society for Microbiology 1993.
A strain representative of Myxococcus virescens has been deposited according to the Budapest Treaty on the International Recognition of the Deposits of Microorganisms for the Purpose of Patent Procedures, on Oct. 4, 1993, at Deutsche Sammlung von Microorganismen und Zellkulturen GmbH Mascheroder Weg 1
REFERENCES:
patent: 4698306 (1987-10-01), Noda
patent: 5116751 (1992-05-01), Shinmen et al.
patent: 5451337 (1995-09-01), Liu et al.
Kato, et al., Plant Cell Physiol. vol. 26, No. 7, pp. 1291-1301 (1985).
Bergey' Manual of Systematic Bacteriology, vol. 3, Staley et al. ed., pp. 2139-2148, 1989.
N.o slashed.rrevang Iben
Pedersen Anders Hjelholt
Sj.o slashed.holm Carsten
Gregg, Esq. Valeta
Marx Irene
Novo Nordisk A S
Zelson Esq. Steve T.
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