Myeloperoxidase assay of endotoxin-induced inflammation

Details Myeloperoxidase assay of endotoxin-induced inflammation Myeloperoxidase assay of endotoxin-induced inflammation

1999-05-03

2000-02-08

Prats, Francisco

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 25, C12Q 128, C12Q 126

Patent

active

060226994

ABSTRACT:
An improved model of endotoxin-induced inflammation is obtained by eliminating the interference in the biochemical assay of myeloperoxidase caused by endogenous reductants and cellular constituents containing free thiol functional groups. The interference was eliminated by 1) extensively diluting soluble, interfering substances and 2) blocking tissue sulfhydril functional groups during tissue homogenization. The improved model can be used to assess the therapeutic potential of anti-inflammatory agents. For example, the model can be used to evaluate topically administrable ophthalmic anti-inflammatory agents in Lewis rats.

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