Mycobacterium primers and probes

Details Mycobacterium primers and probes Mycobacterium primers and probes

1992-07-17

1995-06-06

Wax, Robert A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 34, 536 243, 536 2432, 536 2433, C12Q 168, C12Q 104, C07H 1700

Patent

active

054222427

ABSTRACT:
Primers and probes can be used to detect nucleic acid from Mycobacterium in a sample and determine the species from which the nucleic acid originates. The primers amplify regions of the 16S ribosomal RNA gene and hybridize to regions conserved among species. Genus specific probes hybridize to sequences within the amplified region conserved among mycobacterial species, whereas the species specific probes hybridize to a variable region, so that the species identity can be uniquely determined. Consensus probes for detecting mycobacteria nucleic acids are provided which probes are not identical to any of the sequences of mycobacterial species.

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