Mycobacteriophage specific for the mycobacterium tuberculosis co

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage

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435 6, 435 791, 435 8, 4351721, 4351723, 4353201, 536 2372, C12Q 170, C12Q 168, C12Q 116, C12N 1533

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055829694

ABSTRACT:
Mycobacteriophage DS6A has been characterized and found to specifically infect all species of the TB complex, without any detectable infection of mycobacteria species other than those of the TB complex. DNA sequence analysis revealed several potential open reading frames, including one encoding a protein analogous to gp37 of mycobacteriophage L5 and a second encoding a protein with significant homology to the S. coelicolor DNA polymerase .beta. subunit. Based on the DNA sequence analysis, cloning sites can be identified for insertion of reporter genes, making DS6A useful as a reporter phage for specific detection and identification of species of the TB complex.

REFERENCES:
Bates, in Tuberculosis Current Concepts and Treatment L. N. Friedman Ed., CRC Press pp. 81-92 (1994) "Chapter 5, New Diagnostic Methods".
Jacobs et al. Science 260 819-822 (1993) Rapid assessment of drug susceptabilities of Mycobacterium . . . .
Kotilainen, et al. J. of Bacteriology 175 3089-3095 (1993) Binding of an Escherichia coli double stranded DNA virus PRD1 . . .
Becker et al. Biochimica et Biophysica Acta 264 165-170 (1972) Inactivation by avidin of biotin-modified bacteriophage.

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